杨梅果实EST文库构建及果实品质相关基因表达分析
首发时间:2012-11-15
摘要:以"荸荠"杨梅四个不同成熟度的果实为材料,构建了四个cDNA文库,并分别随机挑选500个克隆进行测序。对这2000条测序得到的表达序列标签(EST)序列进行拼接共获得了395个Unigene,基于Uniprot数据进行功能注释,发现这些Unigene可归为14类。对表达丰度最高的10个Unigene进行了BLAST分析,发现有基因涉及胁迫响应以及花青苷合成。分析了果实成熟衰老和品质调控相关基因在果实成熟期间的表达,并对其可能的作用进行了讨论。
关键词: 杨梅 果实 表达序列标签(EST) 果实成熟衰老 果实品质
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Construction of Chinese Bayberry Fruit EST Library and Expression Analysis of Fruit Quality Associated Genes
Abstract:A total of 2000 expressed sequence tags (EST) were produced from cDNA libraries generated from Chinese bayberry (Myrica rubra Sieb. et Zucc. cv 'Biqi') fruit at four ripening stages. After cluster and assembly analysis of the datasets by UniProt, 395 unigenes were identified and their putative functions were assigned to 14 putative cellular roles. Further sequence BLAST was done for the top ten high frequency genes in the ESTs, and genes associated with disease/defense and anthocyanin accumulation were identified. Expression of genes associated with fruit ripening and senescence as well as fruit quality formation during fruit ripening were analyzed and their possible roles were discussed.
Keywords: Chinese bayberry (Myrica rubra Sieb & Zucc.) fruit expressed sequence tags (EST) fruit ripening and senescence fruit quality
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