BnTst-1, A Tourist-like MITE transposon closely associated with genes and may play important roles in gene expression and species evolution in Brassica species
首发时间:2012-11-22
Abstract:In this paper, a Tourist-like MITE BnTst-1 was identified in Brassica A and C genome. The BnTst-1 copy number and its distribution in different chromosomes in the genome of three Brassica species were investigated. The sequence variation of BnTst-1 and the TIR and TSD were compared among two Brassica species. The amplification profile diversity caused by BnTst-1 activity intra- and inter- species were confirmed experimentally. DNA methylation level of the BnTst-1 and its flanking sequences, together with EMSA experiments showed that this MITE may play regulation role in gene expression and genome evolution in Brassicaea.
keywords: Tourist-like MITE gene expression evolution Brassica
点击查看论文中文信息
MITE转座子BnTrst-1在芸薹属物种基因表达和进化中的作用分析
摘要:本研究在芸薹属"A"和"C"基因组鉴定出一类Tourist-like MITE类转座子。分析了在芸薹属不同种中的拷贝数及分布规律,同时比较了BnTst-1序列两端TIR 和 TSD序列的保守性,找到了芸薹属BnTst-1 转座子的保守区域。进一步的实验证明BnTst-1序列可以造成芸薹属内和属间物种的变异。DNA甲基化分析和凝胶阻滞实验(EMSA)证明了这类转座子在芸薹属基因表达和进化中起着重要的作用。
关键词: Tourist-like MITE 转座子 芸薹属 基因表达 进化
基金:
论文图表:
引用
No.****
同行评议
勘误表
MITE转座子BnTrst-1在芸薹属物种基因表达和进化中的作用分析
评论
全部评论0/1000