超高压对副溶血弧菌细胞壁膜损伤的研究
首发时间:2012-11-01
摘要:目的:研究超高压处理对副溶血弧菌细胞壁膜的损伤效应。方法:本研究以副溶血弧菌为对象,探讨不同压力和保压时间对其存活量的影响,分析超高压处理对其细胞壁膜超微结构,细胞膜的通透性、流动性和Na+/K+-ATP酶活性,细胞壁特性以及细胞表面电位的影响。结果:研究表明,20℃经200 MPa压力作用10 min,副溶血弧菌致死率为100%。超高压处理会对副溶血弧菌细胞壁膜形态结构造成明显的损伤,细胞壁局部破坏、出现缺口;细胞结构不完整、细胞膜消失;由于细胞壁膜的损伤使得胞质内含物出现泄漏。超高压处理会使副溶血弧菌细胞膜通透性异常增加、膜流动性的显著下降、细胞膜Na+/K+-ATP酶活性降低以及细胞壁特性的改变。高于200 MPa压力后,细胞表面zeta电位值随压力的增大而趋向稳定,细胞表面粒子完全絮凝,说明细胞结构已完全被高压破坏。结论:超高压处理会对副溶血弧菌细胞壁膜形态结构造成明显的损伤,改变细胞膜和细胞壁的结构与特性,最终导致其胞内容物和无机盐外漏而致死。
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Effect of Ultra High Pressure on the Cell Wall and Membrane Damage of Vibrio Parahaemolyticus
Abstract:Objective: We demonstrated the effect and potential mechanism of ultra high pressure on the cell wall and membrane damage of Vibrio parahaemolyticus. Methods: The pressure power and time on contribution to the death of Vibrio parahaemolyticus cells were investigated. The effects of ultra high pressure on the microstructural damage of cell wall and membrane, membrane permeability, fluidity and Na+/K+-ATPase activity were examined. Moreover, we explored the characteristic changes of cell wall and cell surface potential of Vibrio parahamolyticus treated by ultra high pressure. Results: No viable cell counts were detected by pressure treatment at 200 MPa and 20 C for 10 min. High pressure treatment caused cell wall and membrane damage in the microstructure of Vibrio parahamolyticus, such as the cell wall breached, cell membrane disappeared, and cytoplasm leaked out. In addition, the enhanced level of membrane permeability, decrease of membrane fluidity and Na+/K+-ATPase activity as well as the characteristic changes of cell wall also attributed to the high pressure treatment. The cell surface zeta potential remained at a relatively stable level after 200 MPa pressure, indicating that the particles appeared on the surface were completely aggregated and the cell structure was thoroughly destroyed by the high pressure. Conclusion: This study indicated that ultra high pressure can cause the microstructural damage, changes of structure and characteristics of cell wall and membrane of Vibrio parahamolyticus, leading to the leakage of cell contents and lethal effect.
Keywords: Food safety ultra high pressure Vibrio parahamolyticus cell wall and membrane damage
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