基于SSR分子标记的9个北沙柳居群遗传多样性
首发时间:2012-12-18
摘要:采用SSR标记的方法对9个居群的266份北沙柳种质资源进行了遗传多样性分析,结果表明:12对SSR引物扩增得到73条带,多态性百分率为86.3%,每对引物扩增位点数2~18,多态信息含量为60.27%~72.60%;Shannon′s信息指数平均为0.2687;根据遗传距离将9个北沙柳居群分为两组。居群间的遗传相似系数(GS)变异范围为0.9318~0.9846,居群间存在一定的遗传变异。北沙柳无性系之间的遗传相似系数范围为64%~95%,伊金霍洛旗扎萨克镇门克庆嘎查(B)、乌审旗查汗淖尔社(C)和呼拉胡社(G)居群中,无性系间存在广泛的遗传变异,具有开展选择育种的潜力。9个居群内无性系为同一母株的克隆分株的可能性较小,收集的无性系独立性较高。
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Genetic Diversity of nine Populations of Salix psammophila based on SSR Molecular Markers
Abstract:Analyzed of genetic diversity of 266 individuals of nine populations of Salix psammophila by SSR Markers,The results show that there are totally 73 bands amplified by twelve pairs of SSR primers and percentage of polymorphic band is 86.3%,the result of genetic distance shows that nine populations of Salix psammophila are divided into two groups. The genetic similarity(GS) among nine populations ranged from 0.9318 to 0.9846, among the populations of some genetic variation.The genetic similarity cofficient between Salix psammophila clone ranged from 64%-95%,the genetic variation of clones groups exist widely in between MenKeQin population in YiJinHuoLuoQi(B),ChaHanNaoErShe(C)and HuLaHuShe(G)in WuShenQi with its has the potential of selection breeding.Clones groups of nine populations of Salix psammophila independence is higher,they are the clonal ramets of the same parent ramet that is low probability.
Keywords: Salix psammophila SSR molecular markers Genetic diversity Germplasm resources
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