银杏EST-SSR引物开发及其评价
首发时间:2012-12-18
摘要:从NCBI的dbEST数据库中下载21604条银杏EST序列,经去冗余处理和SSR位点搜索,共得到2122条EST-SSR序列,利用TRA1.5软件共成功设计1926对SSR引物,随机选取其中100对引物进行合成和筛选,共选出22对具有多态性扩增条带的引物。22对引物在50个银杏品种上共扩增出78个条带,其中多态性条带34条。本研究为SSR标记在银杏相关领域的应用提供了基础。
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Development and evaluation of ginkgo EST-SSR primer
Abstract:In this paper, 21604 EST sequences of ginkgo were downloaded from NCBI dbEST database, and a total of 2122 EST-SSR sequences were aquired after the redundancy processing and SSR loci searching,basing on aboved EST-SSR sequences, we have successfully designed 1926 pairs of primers using TRA1.5 software, among them 100 pairs of primers were randomly selected, synthesized and screened, 22 pairs of primers have amplified polymorphic bands. On 50 varieties of Ginkgo, 22 pairs of primers altogether amplified 78 bands including 34 polymorphic bands. this study has provided a basis for application of SSR markers in related fields of ginkgo.
Keywords: ginkgo biloba EST-SSR primer developing
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