Cellular uptake of aqueous QDs probed by fluorescence correlation spectroscopy
首发时间:2012-12-18
Abstract:Aqueous quantum dots (QDs) directly synthesized with various thiols ligands have been investigated as imaging probes in living cells. However, the effect of its surface chemistry caused by these ligands on QDs' cellular uptakes and its intracellular fate remains poorly understood. In this work, four CdTe QDs were directly synthesized under aqueous conditions using four different thiols as stabilizers, and fluorescence correlation spectroscopy (FCS), X-ray photoelectron spectroscopy (XPS) and Zeta potential measurements on QDs primarily show that the surface structure of these QDs is highly dependent on the thiols ligands used in the preparation of QDs' precursors, including its layer thicknesses, densities, and surface charges. Subsequently, FCS integrated with the maximum-entropy-method-based FCS (MEMFCS) was used to investigate the concentration distribution and dynamics of these QDs in living A-427 cells. It was further verified that, influenced by their different surfaces, QDs showed a very different behavior for cellular membrane adsorption and subsequent internalization. More critically, we show that the cellular uptake of aqueous QDs is dependent on their hydrodynamic diameter and has the potential to escape trapped environments to accumulate in the cytoplasm.
keywords: fluorescence correlation spectroscopy aqueous QDs live cells
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荧光相关光谱研究水相合成量子点的活细胞内吞机理
摘要:采用巯基配体水相合成的量子点已广泛地用于活细胞的成像研究。但对表面配体对量子点内吞的影响的了解却知之甚少。本文采用四种不同的巯基配体合成了四种不同量子点。采用荧光相关光谱(FCS),XPS和zeta电位仪对量子点的表面结构进行了系统研究。研究发现,量子点的表面结构(包括配体层厚度 ,密度和表面电荷等)取决于选用的配体类型。随后,采用FCS和MEMFCS技术对不同量子点在细胞膜和细胞内的浓度分布和扩散时间分布进行了系统研究。研究发现,不同量子点在细胞膜和细胞中呈现不同的吸附和内吞特性。特别是,这些量子点的细胞内吞直接取决于量子点的水合动力学粒径。
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