Colorimetric detection of amplified target DNA by loop-mediated isothermal amplification using gold nanoparticle

• 1、Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, China

Abstract：We have developed a rapid, simple, and label-free colorimetric method for the identification of target DNA. It is based on loop-mediated isothermal amplification (LAMP). Plain gold nanoparticles (AuNPs) are used to indicate the occurrence of LAMP. Its products are mixed with AuNPs in an optimized ration, upon which the deoxyribonucleotides (dNTPs) bind to the AuNPs via ligand-metal interactions and thus enhance their stability. If a target DNA is amplified, the vast reduction of the dNTPs leads to the aggregation of AuNPs and a color change from red to blue. The success of the method strongly depends on the ionic strength of the solution and the initial concentration of dNTPs. Unlike other methods for identification of isothermal products, this method is simple and can be readily applied at sites where instrumentation is inadequate or even lacking.

keywords： label-free gold nanoparticle loop-mediated isothermal amplification naked eyes.

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