Analysis of Ghrelin and growth hormone secretagogue receptor expressions in mouse thymic epithelial cells
首发时间:2012-12-21
Abstract:The present study was to identified the expression of Ghrelin and GHS-R in mouse thymic medullary-type epithelial cells (MTEC1).. Whether the gene of Ghrelin and GHS-R are expressing in MTEC1 cells or not was measured by using quantitative real time RT-PCR (Q-PCR). Results identified that the mRNA of Ghrelin and GHS-R can be detected in MTEC1 cells. The protein levels of GHS-R and ghrelin precursor were detected by the method of western blot analysis. Moreover, the location of GHS-R in MTEC1 cells were detected by immunofluorescence and the quantity of ghrelin in the supernatant of MTEC1 cells were measured by ELISA. The results of western blot analysis make sure that GHS-R and precursor of ghrelin are expressing in MTEC1 cells. GHS-R is a kind of transmembranes protein. as the results of immunofluorescence shown The quantity of ghrelin in the supernatant of MTEC1 cells measured by ELISA was 39.20 pg/ml.
keywords: Ghrelin GHSR MTEC1 cells gene expression
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Ghrelin及GHSR在小鼠胸腺上皮细胞的表达分析
摘要:本文应用实时荧光定量PCR和western blot方法研究表明, Ghrelin及GHSR的mRNA和蛋白质能够表达于体外培养的小鼠胸腺髓质上皮细胞 (MTEC1). 免疫荧光研究发现, GHSR是一种跨膜蛋白.而ELISA 方法测量表明, MTEC1细胞上清液中的Ghrelin含量为39.20 pg/ml.
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