孤雌胚胎干细胞移植缓解心肌梗死后心功能失常和室性重构

刘义; 叶孝颖; 毛丽娜; 程兆康; 姚欣鹏; 贾晓花; 毛铎; 欧来良; 李宗金; 车永哲; 刘娜; 刘林; 孔德领

0
0
浏览
下载

摘要
关键词
基金信息
论文图表
同行评议
相关论文
评论

Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodeling after myocardial infarction

首发时间：2012-12-21

Liu Yi ¹
Liu Yi,Ａ phD student of College of Life Science, Nankai University.
Ye Xiaoying ² Mao Lina ³ Cheng Zhaokang ² Yao Xinpeng ² Jia Xiaohua ² Mao Duo ² Ou Lailiang ² Li Zongjin ⁴ Che Yongzhe ⁵ Liu Na ² Liu Lin ²
Changjiang Scholar, Distinguished Professor of Nankai University, Winner of national outstanding youth fund
Kong Deling ³
Distinguished Professor of Nankai University, Winner of national outstanding youth fund, the director of Key Laboratory Bioactive materials, Ministry of Education

1、State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, TianJin 300071
2、State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071
3、State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, Chinese Academy of Medical Sciences & Peking Union Medical College, Institute of Biomedical Engineering, Tianjin 300192
4、Medical School of Nankai Univeristy, Tianjin 300071
5、Medical School of Nankai University, Tianjin 300071

Abstract：Aims: Parthenogenetic embryonic stem cells (pESCs) are derived from artificially activated oocytes without fertilization and therefore raise minimal ethical concerns and may serve as attractive candidates for regenerative medicine. We thought to investigate whether pESCs can repair myocardial infarction (MI). Methods and Results: Mice (n=89) survived coronary ligation randomly received undifferentiated pESCs, ESCs, or saline. Sham-operated mice received no treatment (n=21). After 7 days, pESCs transplantation promoted pro-angiogenic factors secretion and reduced infiltrated leukocytes. pESCs-treated hearts, superior to ESC group, showed prevented cardiac remodeling and enhanced angiogenesis in and 30 days post MI. Heart contractile function was notably improved by administration of pESCs by 30 days. Moreover, tissues regenerated from the engrafted pESCs in the infarcted myocardium were positive for cardiomyocyte, endothelial cell and smooth muscle cell markers. Furthermore, teratoma fomation appeared in ESCs-treated mice in high proportion (6/34), but surprisingly not found in pESCs-treated mice (0/30) by 30 days. Conclusions: Cardiac dysfunction and adverse ventricular remodeling post MI were attenuated by pESCs transplantation, which may represent an effective and relatively safer strategy for autologous cell therapy in females.

keywords： Parthenogenetic stem cells embryonic stem cell myocardial infarction teratoma stem cell therapy

点击查看论文中文信息

孤雌胚胎干细胞移植缓解心肌梗死后心功能失常和室性重构

刘义 ¹
Liu Yi,Ａ phD student of College of Life Science, Nankai University.
叶孝颖 ² 毛丽娜 ² 程兆康 ² 姚欣鹏 ² 贾晓花 ² 毛铎 ² 欧来良 ² 李宗金 ³ 车永哲 ³ 刘娜 ² 刘林 ²
Changjiang Scholar, Distinguished Professor of Nankai University, Winner of national outstanding youth fund
孔德领 ⁴
Distinguished Professor of Nankai University, Winner of national outstanding youth fund, the director of Key Laboratory Bioactive materials, Ministry of Education

1、药物化学生物学国家重点实验室，生物活性材料教育部重点实验室，天津 300071
2、药物化学生物学国家重点实验室，生物活性材料教育部重点实验室
3、南开大学医学院
4、药物化学生物学国家重点实验室，生物活性材料教育部重点实验室，中国医学科学院北京协和医学院生物医学工程研究所

摘要：目的：孤雌胚胎干细胞（pESC）是胚胎干细胞的一种，免疫原性低且不受伦理限制，但尚无有关pESC移植治疗心肌梗死的研究报道。本实验将pESC和受精卵来源的ESC移植到缺血心肌，探索pESC移植治疗心梗的可行性和安全性。方法：本实验通过永久结扎小鼠左前降支冠脉致心肌梗死，立即将pESC或ESC注射到梗死周边区，盐水组只注射生理盐水，并设假手术组为正常功能对照组。设4个时间点，分析pESC对心肌梗死的疗效。结果：移植pESC能促进缺血区VEGFa、bFGF和IGF-1的表达，减少梗死区浸润的白细胞；植入细胞可表达心肌细胞、内皮细胞和平滑肌细胞标志分子；pESC移植能显著改善心脏功能，减小梗死范围，降低胶原沉积，增强梗死区血管密度；移植到30d时，只ESC移植在心脏部位生成较高比例（6/29）的畸胎瘤；然而移植90d时，pESC组有畸胎瘤发生（2/14）。结论：pESC可发挥旁分泌机制；pESC可再生心肌组织；移植pESC能显著改善心脏功能，有效抑制心室重构；pESC移植安全性较高，但长期移植仍有致畸胎瘤隐患。

关键词：孤雌胚胎干细胞胚胎干细胞心肌梗死畸胎瘤干细胞治疗

基金：

1. Doctoral Fund of Ministry of Education of China （20090031110024）

论文图表：

引用

导出参考文献

.txt

.ris

.doc

Liu Yi,Ye Xiaoying,Mao Lina,et al. Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodeling after myocardial infarction[EB/OL]. Beijing:Sciencepaper Online[2012-12-21]. https://www.paper.edu.cn/releasepaper/content/201212-645.

No.****

同行评议

共计0人参与

全部评论

0/1000

论文编号	201212-645
论文题目	孤雌胚胎干细胞移植缓解心肌梗死后心功能失常和室性重构
文献类型
收录期刊	上传封面中文期刊英文期刊期刊名称（中文）期刊名称（英文）年，卷（）上传封面中文专著英文专著书名（中文）书名（英文）出版地出版社出版年上传封面中文译著英文译著书名（中文）书名（英文）出版地出版社出版年上传封面中文论文集英文论文集编者.论文集名称（中文） [c]. 出版地出版社出版年， - 编者.论文集名称（英文） [c]. 出版地出版社出版年，- 上传封面中文文献英文文献期刊名称（中文）期刊名称（英文）日期-- 在线地址http:// 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期-- 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期--
英文作者写法：中外文作者均姓前名后，姓大写，名的第一个字母大写，姓全称写出，名可只写第一个字母，其后不加实心圆点“.”, 作者之间用逗号“，”分隔，最后为实心圆点“.”, 示例1：原姓名写法：Albert Einstein,编入参考文献时写法：Einstein A. 示例2：原姓名写法：李时珍；编入参考文献时写法：LI S Z. 示例3：YELLAND R L,JONES S C,EASTON K S,et al.