不同物种NRDR表达载体构建及维生素K3催化活性检测
首发时间:2013-01-24
摘要:目的:构建体外表达载体,检测不同物种NADP(H)依赖性视黄醇脱氢/还原酶(NRDR)的维生素K3催化活性。方法:构建人、猪、兔、大鼠、小鼠NRDR的原核表达载体Human pDEST17-NRDR、Pig pDEST17-NRDR、Rabbit pDEST17-NRDR、Rat pDEST17-NRDR、Mouse pDEST17-NRDR,诱导表达后检测其对维生素K3催化活性。结果:不同物种NRDR对维生素K3的催化活性差别比较大,其中猪NRDR的催化效能(kcat/Km)最高,其次是兔NRDR,大鼠和小鼠NRDR催化活性相对较低。结论:猪NRDR可能对结构类似维生素K3的醌类化合物具有较强的还原活性。
关键词: NADP(H)依赖性视黄醇脱氢/还原酶 酶活力 维生素K3
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Expressiion and catalytic activity analysis of NRDR derived from different species
Abstract:Objective: To construct the expression vectors of Hum pDEST17-NRDR, Pig pDEST17- NRDR, Rabbit pDEST17-NRDR, Rat pDEST17-NRDR, and Mouse pDEST17-NRDR, and determine their catalytic activity of menadione. Methods: The prokaryotic expression vectors of NRDRs derived from different species were constructed. The catalytic activities of the NRDRs towards menadione were detected. Results: The recombinant NRDRs could efficiently catalyze the reduction of menadione, and pig NRDR exhibited higher catalytic efficiency. Conclusion: To catalyze efficiently the reduction of quinonoids such as menadione may be the new function of NRDR.
Keywords: NRDR catalytic activity menadione
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