The replication of three members of Alphacoronavirus 1 in cultural cells originated from different host animals

• 1、Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, ShangHai 200032
• 2、Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University

Abstract：The replication characters of alphacoronaviruses, including CCoV, FCoV and TGEV, were studied in different original cell lines to understand the abilities of those viruses to cross host barrier. A72, FCWF and ST cells were infected by CCoV, FCoV and TGEV respectively. The samples of cell cultures were collected at different post-infection pionts. Plaque-assay was carried out to detect the virus titers. The configuration of plaques and the CPE induced by three viruses were observed. Results: The results showed that those viruses replicated effectively in their natural hosts with typical CPE, bigger and good shape plaques, and highest titers of these viruses appeared at post-infection 12 hours. But in no-natural hosts, those three virues showed different characters. The plaques showed that CCoV and FCoV infected A72 cell more effectively than TGEV. FCoV have a higher virulence to FCWF than CCoV, and the titer of TGEV was the lowest. Distinct discriminations were found in ST cell. FCoV and CCoV could not replicate well in ST cells with lower titers and unformed plaque, indicating that those two viruses could not adapted to ST cell. It proved that the coronaviruses intragenus could easily break the species-barrier of each other, but difficult intergenus.

Keywords： Alphacoronavirus Canine coronavirus Feline coronavirus Transmissible gastroenteritis virus Interspecies infection Replicate curve