内质网应激相关基因mRNA在2型糖尿病合并脂肪肝小鼠肝脏中的表达变化
首发时间:2013-01-29
摘要:目的 探讨内质网应激在高脂饮食2型糖尿病转基因MKR小鼠脂肪肝发生中的作用。方法 高脂饲料诱发MKR鼠形成非酒精性脂肪肝模型,观察肝脏形态变化,检测肝脏甘油三酯和脂肪酸水平,RT-PCR检测小鼠肝脏内质网应激相关基因mRNA表达变化。结果 与正常小鼠比较,MKR组小鼠肝脏GRP78、XBP1、FAS、ACC-α和GSK3β mRNA表达水平有升高趋势(P<0.05);与MKR小鼠比较,高脂饮食MKR小鼠肝脏甘油三脂和脂肪酸水平显著升高,肝脏GRP78、XBP1、CHOP、SREBP1c 、FAS、ACC-α、GSK3β和EDEM1基因mRNA表达水平均增加(P<0.01),而apoB100 mRNA表达水平降低(P<0.01)。结论 ERS参与调节肝脏脂质及apoB100的代谢过程,在转基因2型糖尿病MKR鼠脂肪肝形成中发挥重要作用。
For information in English, please click here
The related gene expressions of endoplasmic reticulum stress in the liver of type II diabetes mice combined with liver steatosis
Abstract: Objective To observe the effect of endoplasmic reticulum stress on the formation of liver steatosis in the type 2 diabetes MKR mice raised by high fat feeds. Method MKR mice were raised by high fat feeds to form liver steatosis. The pathological changes of the liver were examined, and the levels of TG and FFA in the liver were examined too. RT-PCR was used to analyze the related gene expressions of endoplasmic reticulum stress in the liver. Results The gene expressions of GRP78, XBP1, FAS, ACC-α and GSK3β on mRNA level in MKR mice were elevated slightly in contrast with normal mice(P<0.05);The levels of TG and FFA in the liver were increased remarkably, and the gene expressions of GRP78, XBP1, CHOP, SREBP1c, FAS, ACC-α, GSK3β and EDEM1 on mRNA level in MKR mice with liver steatosis were elevated significantly in contrast with MKR mice(P<0.01), while the gene expression of apoB100 was decreased(P<0.01). Conclusion There has a close relationship between the liver steatosis and the involvment of ERS in the lipid metabolism in the liver of MKR mice.?????
Keywords: endoplasmic reticulum stress;diabetes combined with liver steatosis;MKR mice;lipid metabolism?????
基金:
论文图表:
引用
No.****
同行评议
共计0人参与
勘误表
内质网应激相关基因mRNA在2型糖尿病合并脂肪肝小鼠肝脏中的表达变化
评论
全部评论0/1000