Epigenetic regulation mechanism of CYP19A gene on ovary reproductive cycle of Japanese flounder (Paralichthys olivaceus)

• 1、Fisheries College, Ocean University of China, 5 Yushan Road, ShanDong QingDao 266003
• 2、Fisheries College, Ocean University of China, 5 Yushan Road, Qingdao 266003, China

Abstract：The adult female Japanese flounder was obtained in this study. Quantitative PCR was used for detecting the gene expression of CYP19A gene during the reproductive cycle, and bisulphate PCR was performed for described the DNA methylation pattern of CpG rich region in CYP19A gene promoter domain. The result founded the CYP19A gene was expressed in the period of oocyte maturation. In stage Ⅳ was reached the highest level, then decreased in stage Ⅴ, While, the opposite tendency was showing in the DNA methylation level of promoter. This implied that P450aromA enzyme play important role in catalyzing synthesis of estrogen and promotes oocyte maturation; DNA methylation modification of CpG sites in promoter may affect the gene expression. These findings preliminary explain the function of CYP19A gene and the molecular mechanism of expression regulation.

Keywords： Japanese flounder DNA methylation CYP19A gene gene expression promoter