Endurance training time-dependent Expressions of ERRα in Skeletal muscle of Mice

• 1、Sport Science College,Beijing Sport University, Beijing 100084
• 2、Sport Science College,Beijing Sport University,Beijing 100084

Abstract：Estrogen-related receptor α (ERRα) can be activated by transcriptional co-activators to regulate the expression of genes involved in fatty acid oxidation, oxidative phosphorylation and mitochondrial biogenesis. Still we know little about the effects of endurance training on the ERRαexpression. In this study, C57BL/ 6J mice were divided into 4 groups randomly based on the different days （0、7、14 and 28 ）, and 7、14、28 groups were divided into control and training groups randomly. ERRαmRNA and total, nuclear ERRα protein levels in the skeletal muscle were measured by Real-time PCR and Western blotting respectively. The results showed that there were no significant differences in its total protein between training and control group, but ERRα mRNA and the nuclear ERRα protein was much higher than those in the control group during 14 and 28 days. Additionally, the total and nuclear ERRα protein were increased with training days. These findings demonstrate that 14 and 28 days' training can increase ERRα mRNA and the nuclear ERRαprotein level，and ERRαprotein can be cumulative increased with training days.

Keywords： training ERRα energy metabolism muscle