Preparation, Characterization and Application of Lectin-Magnetic Particle Conjugates
首发时间:2013-01-23
Abstract:Magnetic particles have been developed for various applications in the biological and clinical determination. In this study, we synthesized the epoxy-activated magnetic particles to immobilization the lectins. The epoxy-activated magnetic particles were the superparamagnetic supports with a specific surface area of 96.29 m2/g. The each gram of the magnetic particles can be saturated with 186.31±5.16 mg Con A. The lectin-magnetic particle conjugates can isolate glycoproteins specially via the lectin's carbohydrate recognition domain with well reproducibility. This method can be a good tool to be applied in the glycoproteomics research.
keywords: Biochemistrty and molecular biology Expoxy-activated magnetic particles Lectin-magnetic particle conjugates Glycoprotein
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凝集素磁性微粒的制备,表征及其应用
摘要:【目的】制备一种可以用于快速、简便分离各种生物样品中糖蛋白的凝集素磁性微粒复合物,用于糖蛋白质组学的研究。【方法】利用制备的环氧化磁性微粒复合物,通过氨基或者巯基同磁性微粒上的环氧基团的化学反应,将凝集素固定化至磁性微粒的表面,并通过各种表征方法对该反应进行优化。【结果】通过条件优化,制备出了平均粒径为3.50 μm,比表面结为96.29 m2/g且富含环氧基团的磁性微粒。利用该磁性微粒制备的凝集素Con A磁性微粒可用于血清中糖蛋白的分离,并具有很好特异性和重复性。【结论】本方法制备的凝集素磁性微粒能很好的分离生物样品中的糖蛋白,可用于糖蛋白质组学的研究。
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