通过PHA聚合酶异源表达提高酿酒酵母的环境耐受性
首发时间:2013-02-27
摘要:酿酒酵母是发酵工业的重要生产菌株,其环境耐受性直接影响到产品的产量和质量。本研究通过异源表达PHA聚合酶,在酿酒酵母中建立PHA合成路径,研究由PHA合成介导的全细胞调控对提高酿酒酵母环境耐受性的影响。结果表明,PHA聚合酶异源表达后,酿酒酵母在16%和18%乙醇应激下,存活率分别提高了3.3倍和14倍;45℃高温应激后,细胞存活率提高不显著,50℃高温应激后,存活率提高了7倍。说明PHA聚合酶异源表达能够提高酿酒酵母乙醇和高温耐受性。
For information in English, please click here
Enhanced thermotolerance and ethanol tolerance in Saccharomyces cerevisiae by heteroexpression of PhaC
Abstract:Polyhydroxyalkanoate synthase PhaC was heteroexpressed in Saccharomyces cerevisiae. The results showed that the activity of PhaC in INVSC1-pYES2-phaC reached 2.45 U/mg, significantly higher than the controls. The viability of the recombinant S. cerevisiae INVSC1-pYES2-phaC increased by 3.3 and 14 fold respectively than INVSC1-pYES2 (control) after 16% and 18% ethanol challenge, and 7 fold after 50℃ heat shock. It was indicated that the heteroexpression of PHA polymerase could significantly increase heat and ethanol tolerance in S. cerevisae. This study provided new evidence for the technical platform of orientational modification of S. cerevisiae stress tolerance.
Keywords: fermentation engineering Saccharomyces cerevisiae thermotolerance ethanol tolerance
论文图表:
引用
No.****
同行评议
勘误表
通过PHA聚合酶异源表达提高酿酒酵母的环境耐受性
评论
全部评论0/1000