树枝形分子与鸟苷酸分子相互作用的定量分析
首发时间:2013-03-22
摘要:在本项目中,采用核磁共振技术(NMR)对树枝形分子/鸟苷酸(GMP)之间的结合/包裹进行了深入的定量探索。一维核磁谱图中G5树枝形分子最外层的亚甲基质子向低场发生了剧烈的移动,说明树枝形分子表面阳离子伯胺基团与GMP分子中的阴离子磷酸根之间形成了离子对结构。此外,通过一维核磁谱图中树枝形分子内部质子峰的位移变化值大小计算出每一个G5树枝形分子平均能结合107个GMP分子,二者之间的结合常数为17400 M-1。
关键词: 高分子化学与物理;树枝形分子;鸟苷酸;相互作用 定量分析
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Quantitative analysis of the interaction between dendrimer and GMP
Abstract:In this study, the binding/ encapsulation between dendrimers and GMP molecules have been investigated by NMR technique with a quantitative method. 1H NMR spectra showed a significant downfield shift of methylene protons in the outmost layer of the G5 dendrimer, indicating the formation of ion pairs between cationic amine groups of dendrimer and anionic phosphate groups of GMP. The chemical shift titration results showed that the binding constant between G5 dendrimer and GMP is 17400 M-1, and each G5 dendrimer has 107 binding sites for GMP.
Keywords: polymer chemistry and physics dendrimer guanosine monophosphate interaction quantitative analysis
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