Solexa深度测序技术检测miRNA在性成熟猪睾丸中的表达情况

周佳伟; 刘刚; 官凯锋; 马昌萍; 雷彬; 童珂雅; 李家连; 李凤娥

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miRNA profiling in the sexually mature porcine testes determined by Solexa deep sequencing

首发时间：2013-04-11

Zhou Jiawei ¹
Zhou jiawei,(1989-)，male，master,small RNA.
Liu Gang ² Guan Kaifeng ² Ma Changping ² Lei Bin ² Tong Keya ² Li Jialian ² Li Fenge ²
Li Fenge(1976-)，female，professor，animal genetics and genomics

1、Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070
2、Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R. China

Abstract：miRNAs are a large family of small regulatory elements that direct messenger RNA degradation or disrupt mRNA translation. High-throughput Solexa sequencing approach was adopted to expand the repertoire of porcine miRNAs and to explore their roles on spermatogenesis. 237,590 total reads (792 unique reads) representing 65 known mature miRNAs in the miRBase release 14.0 were detected. 116 miRNAs including 12 known miRNAs in the miRBase release 15.0 and 104 novel miRNAs were identified from the un-annotated small RNAs. 466,757 targets for 104 novel miRNAs were predicted by MIREAP. The highly expressed miRNAs in porcine testes (let-7c, let-7f, miR-148a, miR-140* and miR-199b) had a very high rate of SNP mutation, and the most popular SNP was T to G mutation at position 6. Three known miRNAs including miR-514, miR-507, miR-508-5p were significantly differentially expressed between some groups (p<0.05), while two novel miRNAs- DB-m0102 and DB-m0071 were not significantly differentially expressed between all detected groups.

keywords： Pig Solexa deep sequencing miRNA Spermatogenesis Testis

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Solexa深度测序技术检测miRNA在性成熟猪睾丸中的表达情况

周佳伟 ¹
Zhou jiawei,(1989-)，male，master,small RNA.
刘刚 ² 官凯锋 ² 马昌萍 ² 雷彬 ² 童珂雅 ² 李家连 ² 李凤娥 ²
Li Fenge(1976-)，female，professor，animal genetics and genomics

1、华中农业大学，农业部猪遗传育种重点实验室，教育部农业动物遗传育种与繁殖重点实验室，武汉 430070
2、Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R. China

摘要：miRNA是直接使信使RNA降解或抑制mRNA翻译的一类大家族的小调控元件。通过高通量Solexa测序方法去扩大猪的miRNA的数目，并探讨其对精子的作用。运用miRBase14.0进行检测到237590总读数（792特异性的读数），代表了65个已知的成熟的miRNA 。运用miRBase15.0检测到116个miRNA，其中包括12个已知的miRNA 和被确定未注明的小分子RNA中的104个新的miRNA。运用MIREAP预测了这104个新miRNA的466757个靶位点。高表达的miRNA（let-7c，let-7f，miR-148a，miR-140*和miR-199b）在猪睾丸中有一个高概率的SNP突变，最常见的SNP是在6处的一个T到G的突变。3个已知的miRNA包括miR-514和miR-507和miR-508-5p在一些群体中是显著性差异表达的（P <0.05），然而两个新的miRNA-DB-m0102和DB-m0071没有检测到显著性差异。

关键词：猪 Solexa深度测序 miRNA 精子形成睾丸

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Zhou Jiawei,Liu Gang,Guan Kaifeng,et al. miRNA profiling in the sexually mature porcine testes determined by Solexa deep sequencing[EB/OL]. Beijing:Sciencepaper Online[2013-04-11]. https://www.paper.edu.cn/releasepaper/content/201304-251.

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论文编号	201304-251
论文题目	Solexa深度测序技术检测miRNA在性成熟猪睾丸中的表达情况
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