白藜芦醇通过激活sirt1抑制间充质干细胞C3H10T1/2的成脂分化
首发时间:2013-04-12
摘要:目的:研究Ⅲ型组蛋白去乙酰化酶sirt1的激动剂白藜芦醇(resveratrol)对间充质干细胞(MSC)C3H10T1/2增殖和成脂分化的影响,并探讨可能的作用机制。 方法: 采用sirt1的激动剂白藜芦醇处理C3H10T1/2,研究其对C3H10T1/2细胞成脂分化的影响,并选择sirt1的选择性抑制剂EX-527处理C3H10T1/2检测其对C3H10T1/2细胞成脂分化的影响。MTT和流式细胞术分别检测不同浓度的白藜芦醇和EX-527对间充质干细胞C3H10T1/2增殖活性和细胞周期的影响;建立稳定的成脂分化模型,采用白藜芦醇和EX-527联合成脂分化诱导培养基(ADM)分别处理C3H10T1/2细胞14天,油红O染色检测两种药物对其成脂分化的影响。结果与结论: resveratrol对C3H10T1/2细胞周期无明显影响,高浓度的resveratrol抑制细胞增殖明显,抑制细胞的成脂分化;EX-527则抑制细胞周期在G0/G1期,促进细胞成脂分化。
关键词: 成脂分化 sirt1 resveratrol EX-527 C3H10T1/2
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Resveratrol inhibits adipogenic differentiation of mesenchymal stem cell C3H10T1/2 by activate sirt1
Abstract:To investigate the effect of class Ⅲ histone deacetylase sirt1 inhibitor Resveratrol on adipogenic differentiation of MSC C3H10T1/2 as well as the underlying mechanism .Methods:Resveratrol as activator of sirt1 was used to treat C3H10T1/2,and then effect of it on adipogenic differentiation of C3H10T1/2 was explored.EX-527 as specific inhibitor of sirt1 was also used to study effect of it on adipogenic differentiation of C3H10T1/2 .MTT assay and flow cytometry-based cell cycle analysis were used to evaluate effects of resveratrol and EX-527 at different concentrations on cell viability and cell cycle of cultured C3H10T1/2 cells.Stable model of adipogenic differentiation was established .Resveratrol and EX-527 combined with ADM were used alternatively to treat C3H10T1/2 14 days.The influences of serial concentrations of resveratrol and EX-527 on adipogenic differentiation of C3H10T1/2 were determined by oil O staining. Conclusions:There are not any notable effects of resveratrol on C3H10T1/2 cell cycle,but high concentration of resveratrol remarkably restrains proliferation of C3H10T1/2 .Resveratrol exerts negative effect on adipogenic differentiation of C3H10T1/2.EX-527 exerts positive effect on adipogenic differentiation of C3H10T1/2 and cell cycle of C3H10T1/2 is arrested at G0/G1 phase.
Keywords: adipogenic differentiation sirt1 resveratrol EX-527 C3H10T1/2
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白藜芦醇通过激活sirt1抑制间充质干细胞C3H10T1/2的成脂分化
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