Transcriptional analysis of the conidiation pattern shift of entomopathogenic fungus, Metarhizium acridum, in response to different nutrients
首发时间:2013-05-01
Abstract:Most fungi have two different conidiation patterns: normal conidiation and microcycle conidiation under different culture conditions, including the nutrients in the medium. However, the mechanism of conidiation pattern shift response to culture conditions is poorly understood. In this study, Metarhizium acridum conducting microcycle conidiation on sucrose yeast extract agar medium (SYA) shifted to normal conidiation when supplemented with sucrose, nitrate, or phosphate. The M. acridum transcripts on SYA, SYA supplemented with sucrose, nitrate or phosphate, and 1/4SDAY were sequenced by Solexa/Illumina deep-sequencing technology. The results showed that 6,931 genes involved in 243 biochemical pathways were either up- or downregulated in response to different nutrients. Comparative analysis demonstrated that 1,859 genes specifically upregulated at the microcycle conidiation stage were selected. One hundred and twelve, 292, or 216 genes might be associated with microcycle conidiation after adding sucrose, nitrate, or phosphates to SYA medium, respectively. Four hundred and seventy genes were specifically upregulated and might be related to normal conidiation. KEGG pathway analysis indicated that these genes were mainly associated with the cell cycle, cell proliferation, metabolism, protein transport and signal transduction, and the mitogen-activated protein kinase signaling and catabolism pathway. Changing sucrose, nitrate or phosphate, led to a shift between microcycle and normal conidiation by perturbing the cell cycle and metabolism of M. acridum. This research provides essential information about the molecular mechanism of conidiation pattern shift induced by single nutrients.
keywords: Metarhizium acridum conidiation pattern shift pathway analysis Gene expression profiling
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不同营养条件下绿僵菌产孢模式转变的转录本分析
摘要:大多数真菌有两种产孢方式:正常产孢和微循环产孢,昆虫病原真菌绿僵菌也存在这两种产孢方式。改变培养基的营养成分可以使产孢方式发生改变。但是有关这种转变的分子机制尚不明朗。在微循环产孢培养基(SYA)上分别改变碳源、氮源、磷源的含量可以使绿僵菌的产孢方式发生改变。转录本分析在SYA文库和其他4种正常产孢培养基文库中我们共得到6,931个基因,这些基因可以注释到243个通路中,它们通过上调或下调表达来应对不同营养物质对产孢模式转变的调控。通过差减比对分析发现有1,859个基因在微循环产孢时期高表达,470个基因在正常产孢时期高表达,112, 292, 216个基因可能分别参与碳源、氮源、磷源对产孢模式转变过程的调控。KEGG通路分析证明大多数基因与细胞循环、细胞增殖、细胞代谢、蛋白转运及信号转导相关。研究证明在绿僵菌中营养物质通过扰乱细胞循环及代谢过程来控制微循环产孢和正常产孢的。本研究也为进一步研究微循环产孢的机制和营养调控产孢方式转变的机制提供了一种方法。
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