P-糖蛋白介导药物相互作用的研究方法
首发时间:2013-05-13
摘要:P-糖蛋白(P-glycoprotein,P-gp)是由多药耐药基因(MDR)编码,在人体正常组织以及部分肿瘤细胞内表达的膜蛋白,它能够将选择性地将药物从细胞内转运外排出,从而影响药物吸收、分布与排泄。 基于P-gp的药物-药物相互作用(drug-drug interactions, DDI)的研究对定向药物设计,缩短药物开发周期等起到促进作用。关于P-gp的DDI的研究方法主要包括体内法(in vivo)、在体法(in situ)、体外法(in vitro)等。本文就目前常用的P-gp的研究方法及其特点进行综述。
关键词: P-糖蛋白 基因敲出 肠灌流 肝灌流 单层细胞 ATP酶活力 MCF-7/ADR细胞
For information in English, please click here
Methods of P-glycoprotein-mediated drug interactions
Abstract:P-glycoprotein (P-gp) encoded by drug resistant gene and expressed in human normal tissues and in some tumor cells, is a very important factor to limit drug absorption, distribution, excretion, and maybe a factor for some tumor drug treatment failure; Researches on P-gp are very important significant to elucidate the drug-drug interactions (DDI) based on P-gp, can play a catalytic role in designing directed drug, reducing the drug development cycle. We often use different methods to predict drug intestinal absorption and distribution of tissues and organs, including in vivo, in situ and in vitro. In this paper, the current methods for P-gp research are reviewed.
Keywords: P-glycoprotein Gene knockout Intestine perfusion Liver perfusion Cell monolayer ATPase activity MCF-7/ADR cell
论文图表:
引用
No.****
同行评议
勘误表
P-糖蛋白介导药物相互作用的研究方法
评论
全部评论0/1000