独活寄生汤通过加快G1/S期转换促进骨性关节炎软骨细胞增殖

吴广文; 陈文列; 郑春松; 褚建锋; 林如辉; 叶锦霞; 许惠凤; 李西海; 黄云梅; 叶蕻芝; 刘献祥; 吴明霞

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Duhuo Jisheng Decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis

首发时间：2013-07-01

WU Guangwen ¹
WU Guangwen,(1982-),Male, Research associate, Osteoarthritis research.
CHEN Wenlie ² ZHENG Chunsong ² CHU Jianfeng ² LIN Ruhui ³ YE Jinxia ³ XU Huifeng ² LI Xihai ² HUANG Yunmei ³ YE Hongzhi ² LIU Xianxiang ² WU Mingxia ⁴
WU Mingxia(1965-), Female, Professor, Osteoarthritis research

1、Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, FuZhou 350122
2、Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine
3、Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine
4、Second Affiliatted People's Hospital of Fujian University of Traditional Chinese Medicine

Abstract：Objective: To investigate the molecular mechanisms behind the therapeutic effects of Duhuo Jisheng Decoction (DHJSD) on osteoarthritis (OA). Methods: A total of 27 two-month-old male Sprague Dawley rats were randomly divided into three groups: the control group (no papain-induced OA; received an equivalent amount of saline only), the model group (papain-induced OA; received an equivalent amount of saline only) and the DHJSD group [papain-induced OA; received a clinical oral dose of DHJSD (9.3g/kg/day)]. After 8 consecutive weeks of treatment, the morphological changes in articular cartilage were observed under an optical microscopy and by transmission electron microscopy (TEM), and the mRNA and protein expression levels of Cyclin D1, CDK4, CDK6, Rb and p16 were measured by RT-PCR and immunohistochemistry, respectively. Results: Treatment with DHJSD significantly improved the arrangement of articular cartilage structure and collagen fibers and reduced cell degeneration compared with the model group. The mRNA and protein expression levels of Cyclin D1, CDK4, CDK6 and Rb in DHJSD-treated group were significantly increased compared to those in the model group, whereas p16 expression was significantly down-regulated. Conclusion: DHJSD treatment promotes chondrocyte proliferation by promoting the G1/S checkpoint transition in the cell cycle and by up-regulating the expression of Cyclin D1, CDK4, CDK6 and Rb and down-regulating the expression of p16 and this may, in part, explain its clinical efficacy in the treatment of osteoarthritis.

keywords： Orthopedics of Chinese Medicine Duhuo Jisheng Decoction (DHJSD) chondrocyte proliferation cell cycle osteoarthritis

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独活寄生汤通过加快G1/S期转换促进骨性关节炎软骨细胞增殖

吴广文 ¹
WU Guangwen,(1982-),Male, Research associate, Osteoarthritis research.
陈文列 ² 郑春松 ² 褚建锋 ² 林如辉 ³ 叶锦霞 ³ 许惠凤 ² 李西海 ² 黄云梅 ³ 叶蕻芝 ² 刘献祥 ² 吴明霞 ⁴
WU Mingxia(1965-), Female, Professor, Osteoarthritis research

1、福建中医药大学中西医结合研究院，福州 350122
2、福建中医药大学中西医结合研究院
3、福建中医药大学中西医结合研究院医学实验中心
4、福建中医药大学附属第二人民医院

摘要：Objective: To investigate the molecular mechanisms behind the therapeutic effects of Duhuo Jisheng Decoction (DHJSD) on osteoarthritis (OA). Methods: A total of 27 two-month-old male Sprague Dawley rats were randomly divided into three groups: the control group (no papain-induced OA; received an equivalent amount of saline only), the model group (papain-induced OA; received an equivalent amount of saline only) and the DHJSD group [papain-induced OA; received a clinical oral dose of DHJSD (9.3g/kg/day)]. After 8 consecutive weeks of treatment, the morphological changes in articular cartilage were observed under an optical microscopy and by transmission electron microscopy (TEM), and the mRNA and protein expression levels of Cyclin D1, CDK4, CDK6, Rb and p16 were measured by RT-PCR and immunohistochemistry, respectively. Results: Treatment with DHJSD significantly improved the arrangement of articular cartilage structure and collagen fibers and reduced cell degeneration compared with the model group. The mRNA and protein expression levels of Cyclin D1, CDK4, CDK6 and Rb in DHJSD-treated group were significantly increased compared to those in the model group, whereas p16 expression was significantly down-regulated. Conclusion: DHJSD treatment promotes chondrocyte proliferation by promoting the G1/S checkpoint transition in the cell cycle and by up-regulating the expression of Cyclin D1, CDK4, CDK6 and Rb and down-regulating the expression of p16 and this may, in part, explain its clinical efficacy in the treatment of osteoarthritis.

关键词： Orthopedics of Chinese Medicine; Duhuo Jisheng Decoction (DHJSD); chondrocyte; proliferation; cell cycle; osteoarthritis

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1. This study was supported by the Special Research Fund for Doctor Discipline in College （20123519110001）

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WU Guangwen,CHEN Wenlie,ZHENG Chunsong,et al. Duhuo Jisheng Decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis[EB/OL]. Beijing:Sciencepaper Online[2013-07-01]. https://www.paper.edu.cn/releasepaper/content/201307-20.

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论文编号	201307-20
论文题目	独活寄生汤通过加快G1/S期转换促进骨性关节炎软骨细胞增殖
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