牛毛水解物的自由基清除活性
首发时间:2013-07-16
摘要:用碱法水解牛毛制备了牛毛水解物,通过凯氏定氮法确定其蛋白质含量为85.57%。采用不同实验体系(ABTS、DPPH、羟自由基、双氧水)检测牛毛水解物对不同自由基的清除作用,并用普鲁士蓝法测定其对三价铁离子的还原能力。结果表明,牛毛水解物对ABTS、DPPH、羟自由基及双氧水具有显著的清除作用,其半数有效浓度(EC50)分别为3.94mg/mL、3.64mg/mL、2.63mg/mL和26.65μg/mL,对三价铁离子表现出较强的还原能力。
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Free Radical Scavenging Activity of Bovine Hair Hydrolysates
Abstract:Bovine hair hydrolysate was prepared by alkaline hydrolysis of bovine hair from leather industry. The free radical scavenging activity and reducing capacity of the hydrolysate were evaluated by using various testing systems. The hydrolysates exhibited remarkable antioxidant activity in scavenging ABTS, DPPH and hydroxyl radicals, as well as hydrogen peroxide, where the EC50 values were 3.94mg/mL、3.64mg/mL、2.63mg/mL and 26.65μg/mL, respectively. Moreover, the hydrolysate presented high reducing power to Fe+3. Our results suggested that bovine hair could be used as a bioactive protein source for novel usage.
Keywords: Bovine hair keratin hydrolysate of protein free radical scavenging activity
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