High efficiency of neuron differentiation from neural stem cells by monolayer culturing by mash1>
首发时间:2013-07-17
Abstract:Efficient generation of neuroprogenitor or neurons is necessary for the development of autologous cell therapies for the treatment of neurological disease. Neural stem cells (NSCs) represent a valuable resource for both research and regenerative medicine due to their ability to propagate indefinitely in vitro and to spontaneously differentiate into neurons. However, several weeks are often required for neuronal generation, and the percentage of resulting neurons is often relatively low. In this study, we adopted the initiative methods in China. Primary neurosphere monolayer from rat EC stem cells was isolated by selecting a single colony during passage, more than 95% of neural stem cells formed the neurosphere monolayer only after 1 day of differentiation, and purified homogenous NSCs proliferated throughout the entire plate after six passages. This monoclonic culture assay on neurosphere monolayer successfully generated high-contented neuron with high purity. In addition, the effect of serum concentration on neuronal production was also characterized in this study. These novel methods will be valuable for both research purposes as well as for clinical therapies.
keywords: neural stem cells neuroprogenitor neurons serum concentration
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High efficiency of neuron differentiation from neural stem cells by monolayer culturing by mash1>
摘要:Efficient generation of neuroprogenitor or neurons is necessary for the development of autologous cell therapies for the treatment of neurological disease. Neural stem cells (NSCs) represent a valuable resource for both research and regenerative medicine due to their ability to propagate indefinitely in vitro and to spontaneously differentiate into neurons. However, several weeks are often required for neuronal generation, and the percentage of resulting neurons is often relatively low. In this study, we adopted the initiative methods in China. Primary neurosphere monolayer from rat EC stem cells was isolated by selecting a single colony during passage, more than 95% of neural stem cells formed the neurosphere monolayer only after 1 day of differentiation, and purified homogenous NSCs proliferated throughout the entire plate after six passages. This monoclonic culture assay on neurosphere monolayer successfully generated high-contented neuron with high purity. In addition, the effect of serum concentration on neuronal production was also characterized in this study. These novel methods will be valuable for both research purposes as well as for clinical therapies.
关键词: neural stem cells; neuroprogenitor; neurons; serum concentration
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High efficiency of neuron differentiation from neural stem cells by monolayer culturing by mash1>
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