Preliminary Study of Skeletal muscle satellite cells and glioma cells cultured

• 1、Life Sciences School, Nantong University, Nantong, Jiangsu, 226019

Abstract：????? Objective ：The SD rat skeletal muscle satellite cells were investigated in primary culture and identification methods, and rat glioma cell lines were cultured in vitro and determined for their biological properties. Methods ：The bilateral gastrocnemius and vastus intermedius muscle in SD rats of 10 weeks after birth under sterile conditions were cut, using primary tissue culture and subculture, differential adherent culture and purification of skeletal muscle satellite cells, immunofluorescence cell chemical to be identified. And trypsin digestion method was implemented for rat glioma cell lines in vitro culture. Results ：The fast and slow skeletal muscle satellite cells are applied in primary and purification spread 2-5 generations, fibroblasts excluded, the immunocytochemistry fluorescent antibody of Desmin, Fast-myosin, Slow-myosin was implemented to be separately identified. And the rat glioma cells in vitro culture were used to determine its biological morphology. Conclusion ：The skeletal muscle satellite cells in primary culture, purification and identification technology were established. And rat glioma cell passage way for the future of skeletal muscle satellite stem cells and rat glioma cells in-depth study was established foundation.

Keywords： animal cytology skeletal muscle satellite cells glioma cells primary culture subculture?????