慢乙肝脾胃湿热证转录组学的PCR验证研究
首发时间:2013-10-18
摘要:目的 对前期慢性乙型肝炎脾胃湿热证转录组学研究结果进行的PCR验证。方法 前期转录组学研究发现慢性乙型肝炎脾胃湿热证的差异基因(差异倍数在2倍以上)有LOC440040、SYCP2L、MYH6、MDGA1、C17orf97、LIPC、ATOH8等,本研究借助荧光定量PCR技术对上述基因进行定量检测。结果 慢乙肝脾胃湿热证患者 C17orf97、LIPC、ATOH8的mRNA表达水平显著降低,差异有统计学意义(P<0.05,P<0.01),而慢乙肝脾胃湿热证患者LOC440040、MDGA1、SYCP2L、MYH6的mRNA表达水平显著升高,差异有统计学意义(P<0.01)。结论 前期转录组学研究发现慢性乙型肝炎脾胃湿热证的差异基因(差异倍数在2倍以上)LOC440040、SYCP2L、MYH6、MDGA1、C17orf97、LIPC、ATOH8等,结果可靠,可以作为慢乙肝脾胃湿热证客观化的标志物之一。
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Study on Verifing the Transcriptome Result of Spleen-Stomach Damp-Heat Syndrome of Chronic Hepatitis B by PCR
Abstract:Objective We tested prior transcriptome results of Spleen-Stomach Damp-Heat Syndrome of Chronic Hepatitis B by PCR.Methods Preliminary transcriptome study revealed that differentially expressed genes of Spleen-Stomach Damp-Heat Syndrome of Chronic Hepatitis B (fold difference in the two times) included LOC440040, SYCP2L, MYH6, MDGA1, C17orf97, LIPC, ATOH8 etc, We quantitatively detected the gene by quantitative PCR.Results C17orf97, LIPC, ATOH8 mRNA expression level were significantly lower in Chronic Hepatitis B patient with Spleen-Stomach Damp-Heat Syndrome, the difference was statistically significant (P <0.05, P <0.01), but LOC440040, MDGA1, SYCP2L, MYH6 mRNA expression level were significantly increased, the difference was statistically significant (P <0.01).Conclusion Preliminary transcriptome study revealed that differentially expressed genes of Spleen-Stomach Damp-Heat Syndrome of Chronic Hepatitis B (fold difference in the two times) includes LOC440040, SYCP2L, MYH6, MDGA1, C17orf97, LIPC, ATOH8 etc, which is reliable to be used as one of the objectified markers for Spleen-Stomach Damp-Heat Syndrome of Chronic Hepatitis B.
Keywords: Spleen-Stomach Damp-Heat Syndrome,Transcriptome,PCR validation
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