Changes of proteins induced by anticoagulants can be more sensitively detected in urine than in plasma
首发时间:2013-11-15
Abstract:Biomarker studies commonly monitor the composition of plasma, which is under strict homeostatic control, while urine is not. Urine is a partial filtrate of blood; however, its composition reflects systemic physiology. Here we show that changes in the coagulation status of blood can be more sensitively detected in urine than in plasma. Plasma and urine protein composition was analysed by LC-MS/MS and Western blot in six SD female rats before and after treatment with heparin or argatroban. LC-MS/MS and Western blot analyses demonstrated drug-induced increases in transferrin and hemopexin levels in urine but not in plasma. Our data indicates that urine may serve as a source for more sensitive detection of protein biomarkers than plasma.
keywords: bioscience biomarker urine plasma proteomics
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抗凝剂引起的蛋白变化可以在尿中更敏感的监测
摘要:生物标志物是和一个生理或者病理生理过程相联系的可监测的变化,最常用于检测生物标志物的体液是血液。但血液有严格的稳态调节,理论上不允许较大的变化持续存在。尿液则没有稳态的限制,并且尿液的一部分是血液滤过物,它在一定程度上可以反映整个机体的状态。本实验通过不同抗凝剂(肝素或阿加曲班)改变雌性SD大鼠的凝血状态,检测给药前后血浆和尿液中蛋白的改变。在同样的分析条件下,尿液中发现更多的变化蛋白。肝素和阿加曲班组尿液中变化的蛋白分别为有27和61个,相应的血液中仅有3和1个。两个变化的蛋白Transferrin和hemopexin进一步用western blot得到验证。本实验提示尿液是更敏感的蛋白生物标志物来源。
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