家蚕的抗农药能力检测
首发时间:2013-12-06
摘要:野蚕经过长期的自然选择和我们的祖先五千多年的人工驯化,逐渐成为家养动物-家蚕。相对于野蚕,家蚕对有机磷和菊酯类农药更加敏感,对农药和环境抗逆性差,因此可能成为抗农药能力检测的指示生物。本实验通过测定家蚕和野蚕体内乙酰胆碱酯酶活性,来评价其抗农药能力。从测定结果看,家蚕的乙酰胆碱酯酶活性比野蚕的乙酰胆碱酯酶活性低,说明野蚕具有更强的分解乙酰胆碱能力,表现出对有机磷和菊酯类农药更高的抵抗性。而家蚕则对有机磷和菊酯类农药很敏感。
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Detection on resistant capability of silkworm to pesticides
Abstract:The wild silkworm was becoming domesticated animals - the silkworm after a long time of more than five thousand years' artificial domestication and breeding by our ancestors. Compared to the wild silkworm, the domesticated silkworm has a low stress resistance to the pesticides and the environment,which become one of the ideal biological indicators to study the pesticides or insecticides resistance.This article studied the activities of acetylcholinesterase (AChE) in wild and domesticated silkworm to evaluate the resistant capability of silkworm to pesticides. The results showed that AChE activity in domesticated silkworm was lower than the wild silkworm. This indicated that wild silkworm have a stronger ability to break down acetylcholine, showing higher resistance to organophosphate and pyrethroid pesticide. On the contrary, the domesticated silkworm is very sensitive to organophosphate and pyrethroid pesticides.
Keywords: domesticated silkworm;wild silkworm Acetylcholinesterase(AChE) resistance to the pesticides biological indicator
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