Study of soothe the liver and regulate Qi and activating blood method combined with bone marrow stem cells on myocardial cell apoptosis and related gene expression effect in myocardial ischemia reperfusion injury ( IRI ) rats

• 1、College of traditional Chinese medicine ，Hunan University of Chinese Medicine，Changsha 410208

Abstract：Objective To study of soothe the liver and regulate Qi and activating blood method combined with bone marrow stem cells on myocardial cell apoptosis and related gene expression effect and mechanism in myocardial ischemia reperfusion injury ( IRI ) rats. Methods Preparation of myocardial IRI model, through the BMSCs transplantation in IRI rats methods, were randomly divided into sham operation group（A）; IRI group（B）; BMSCs group; D joint group（D）. Experiment were observated by the end of probe labeling method for the detection of myocardial cell apoptosis, by immunohistochemistry observation of myocardial cells in Bcl-2, Bax protein expression. Results Compared with group BMSCs, the combined group of myocardial cell apoptosis index was lower, the difference was significant ( P < 0.01 ). The combined group elevated Bcl-2 expression, the expression of Bax was significantly decreased, with a significant difference ( P < 0.05 ). Conclusions Soothe the liver and regulate Qi and activating blood method can inhibit bone marrow stem cell transplantation in IRI rat myocardial cell apoptosis, promote myocardial regeneration.

Keywords： Internal medicine of traditional chinese medicine Soothe the liver and regulate Qi and activating blood method；Bone marrow stem cells； ischemia reperfusion injury；myocardial cell apoptosis