E47与PAK5在肿瘤细胞内相互作用的验证
首发时间:2013-12-06
摘要:目的:验证E47与PAK5在肿瘤细胞内的相互作用。方法:用GST-pull down实验鉴定E47和PAK5在体外的相互作用;免疫共沉淀实验确定E47和PAK5在肠癌细胞SW480细胞内的相互作用;共焦激光扫描显微镜观察E47和PAK5在肠癌细胞HCT116中的定位及共定位。结果:E47和PAK5能够在体外直接结合;E47和PAK5能够在肠癌细胞SW480内相互作用;E47和PAK5在肠癌细胞HCT116中共定位。结论:E47和PAK5能在肿瘤细胞内相互作用。
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Identification of E47 Interaction with PAK5 in tumor cells
Abstract:OBJECTIVE: Confirm the interaction between E47 and PAK5. METHODS: Interaction between E47 and PAK5 in vitro was examined by GST-pull down. Interaction bwtween PAK5 and E47 in colon cancer cell SW480 was identified by Co-IP. Immunofluorescence was used to observe the localization and co-localization of E47 and PAK5 in colon cancer cell HCT116. RESULTS: Interaction between E47 and PAK5 in vitro was confirmed. Association of E47 and PAK5 in colon cancer cell SW480 was identified. Co-localization of E47 and PAK5 was observed in colon cancer cell HCT116. CONCLUSIONS: E47 interactacted with PAK5 in colon cancer cell.
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