Development of a small alien-segment translocation line carrying the HMW-GS gene of Dasypyrum villosum

• 1、National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute,Nanjing Agricultural University, NanJing 210095

Abstract：Glu-V1 locus, located in the chromosome 1V of Dasypyrum villosum, was proved to have positive effects on grain quality. However, there are very few reports about the transfer of the D. villosum-derived HMW-GS gene into wheat background by chromosome manipulation. In the present study, a small alien-segment translocation line NAU1V-3-5 was developed through ionizing radiation of the mature female gametes of CS - D. villosum 1V disomic addition line and confirmed by cytogenetic analysis. Genomic in situ hybridization, chromosome C-banding and 1V-specific EST-STS markers enabled the translocated chromosome was T1VS-6BSo6BL. And seed storage protein analysis confirmed the Glu-V1 allele of D. villosum was Glu-V1a and its coded protein was V71 subunit. The T1V#4S-6BSo6BL translocation line could be useful germplasms for bread wheat improvement. The developed 1VS-specific molecular markers could be used to rapidly identify and trace the alien chromatin of 1VS in wheat background.

Keywords： Dasypyrum villosum small alien-segment translocation line HMW-GS