Study on the Interaction Characteristics of Lomefloxacin and/or Cefazolin with Bovine Serum Albumin by Spectroscopic Technique
首发时间:2014-01-23
Abstract:In this paper, the interaction of lomefloxacin (LMF) and/or cefazolin (CFZ) with bovine serum albumin (BSA) was studied by fluorescence quenching in combination with UV-Vis spectroscopic method under simulative physiological conditions. The fluorescence quenching constants, binding distance, and binding constants for BSA-LMX and/or CFZ systems were determined. The fluorescence quenching of BSA by addition of LMF and/or CFZ is due to static quenching and energy transfer. The ratio of binding constants (KA) for BSA-LMF to BSA-CFZ equals to 56.8. In the presence of CFZ (LMF), the binding distance of BSA-LMF (BSA-CFZ) decreased from 4.44 to 2.45 nm (from 1.94 to 1.48 nm), and the binding constant (KA) of BSA-LMF (BSA-CFZ) increased from 2.87×105 to 5.43×105 L/mol (from 5.15×103 to 1.24×104 L/mol). Two-coexisting CFZ and LMF may lead to the need for more doses to achieve therapeutic effect. Circular dichroism spectra, synchronous fluorescence, and three-dimensional fluorescence studies showed that the presence of LMF or CFZ could change the conformation of BSA during the binding process, and the presence of coexisting LMF and CFZ could change the conformation of BSA further, here LMF was reigning.
keywords: Fluorescence Bovine serum albumin Lomefloxacin Cefazolin Interaction characteristics
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荧光法研究牛血清白蛋白存在下洛美沙星与头孢唑林钠间相互作用
摘要:利用荧光光谱法结合紫外光谱法,在模拟人体生理条件下研究了洛美沙星(LMF)及头孢唑林钠(CFZ)分别单独与牛血清白蛋白(BSA)、头孢唑林钠和洛美沙星同时与牛血清白蛋白等的相互作用。两种药物单独与牛血清白蛋白的相互作用结果表明均为静态猝灭,测定了各种体系的荧光猝灭常数、结合常数和结合距离。体系BSA-LMF与体系BSA-CFZ的结合常数之比为56.8。在CFZ(LMF)存在下,BSA-LMF(BSA-CFZ)体系的结合距离从4.44下降到2.45 nm (从1.94下降到1.48 nm), BSA-LMF(BSA-CFZ)体系的结合常数从2.87×105增加到5.43×105 L/mol (从 5.15×103 增加到1.24×104 L/mol)。两种药物的共存导致二者间的拮抗作用。圆二色谱、同步荧光光谱和三维荧光光谱研究显示出洛美沙星或者头孢唑林钠单独作用均能改变牛血清白蛋白的构象,二者共存时进一步改变牛血清白蛋白的构象,其中洛美沙星起主要影响作用。
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荧光法研究牛血清白蛋白存在下洛美沙星与头孢唑林钠间相互作用
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