E3泛素连接酶HERC4在膀胱癌组织中的表达
首发时间:2014-01-13
摘要:目的 研究HERC4在人膀胱癌组织和癌旁正常膀胱上皮组织中的差异表达。方法 Real-time PCR法检测40对膀胱癌组织标本和癌旁正常组织标本中HERC4的mRNA表达水平; Western blotting和免疫组化法检测配对标本中HERC4的蛋白表达水平。结果 Real-time PCR结果显示HERC4的mRNA的表达水平在膀胱癌组织中较癌旁正常组织高 (P<0.05)。Western blotting和免疫组化结果显示HERC4蛋白表达差异与mRNA表达差异趋势一致(P<0.05)。染色结果显示HERC4主要在细胞质中表达,膀胱癌组织中HERC4阳性表达率为55.7%,癌旁正常膀胱上皮组织为 17.6%。结论 HERC4与膀胱癌的发生有关,并有可能作为膀胱癌的早期诊断标志物和治疗靶点。
关键词: 膀胱癌 HERC4 Real-time PCR Western blotting 免疫组织化学
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Expression of E3 ligase HERC4 in bladder cancer tissues
Abstract:To investigate the expression difference of HERC4 between human bladder cancer tissues and adjacent normal bladder tissues. Methods: Real-time PCR was used to detect mRNA expression of HERC4 in 40 cases of bladder cancer tissues and paired normal bladder tissues,western blotting and immunohistochemical method were applied to detect protein expression of HERC4 in the paired tissue samples. Results: The results of real-time PCR showed significantly higher mRNA expression of HERC4 in bladder cancer tissues than in normal bladder tissues (P<0.05). Western blotting and immunohistochemistry demonstrated that HERC4 protein expression difference in line with mRNA expression difference between cancer and normal tissues (P<0.05). Immunohistochemistry indicated HERC4 expression was located majorly in the cell cytoplasm. Positive expression were detected in 55.7% of the bladder cancer tissues as compared to 17.6% in the adjacent normal tissues.Conclusion HERC4 is correlate with the tumorigenesis of bladder cancer and may be used as a new potential biomarkers for early diagnosis and therapeutic target in bladder cancer.
Keywords: Bladder cancer HERC4 Real-time PCR Western blotting Immunohistochemistry
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