依达拉奉对大鼠短暂性全脑缺血后海马CA1区的保护作用及对颗粒下区神经发生的影响

雷珊; 张蓬勃; 李卫松; 高明; 贺西京; 郑娟; 李旭; 王宁; 汪晓; 张军峰; 祁存芳; 吕海侠; 陈新林; 刘勇

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Pre- and post-treatment with edaravone protects CA1 hippocampus and enhances neurogenesis in the subgranular zone of dentate gyrus after transient global cerebral ischemia in rats

首发时间：2014-01-16

LEI Shan ¹
LEI Shan,1986.07,female,doctoral student,the neuroprotection of cerebral ischemia
ZHANG Pengbo ²
ZHANG Pengbo,1968.03,male,professor,the neuroprotection and regeneration of cerebral ischemia
LI Weisong ² GAO Ming ² HE Xijing ³ ZHENG Juan ² LI Xu ² WANG Ning ² WANG Xiao ² ZHANG Junfeng ⁴ QI Cunfang ⁴ LV Haixia ⁴ CHEN Xinlin ⁴ LIU Yong ⁴

1、 Department of Anesthesiology, Second Affiliated Hospital of Xi’an Jiaotong University School of Medicine, 157# West 5 road, Xi’an 710004
2、Department of Anesthesiology, Second Affiliated Hospital of Xi’an Jiaotong University School of Medicine, 157# West 5 road, Xi’an 710004
3、Department of Orthopedics, Second Affiliated Hospital of Xi’an Jiaotong University School of Medicine, 157# West 5 road, Xi’an 710004
4、Institute of Neurobiology, National Key Academic Subject of Physiology of Xi’an Jiaotong University School of Medicine, 76# Yanta West Road, Xi’an 710061

Abstract：Edaravone is clinically used for treatment of patients with acute cerebral infarction. However, the effect of edaravone on neurogenesis in the hippocampus following ischemia remains unknown. In the present study, we explored whether pre- and post- treatment of edaravone had any effect on neural stem/progenitor cells (NSPCs) in the subgranular zone (SGZ) of hippocampus in a rat model of transient global cerebral ischemia. Male Sprague-Dawley rats were divided into 3 groups, sham-operated (n = 15), control (n = 15), and edaravone-treated (n = 15) groups. Newly-generated cells were labeled by 5-bromo-2-deoxyuridine (BrdU). Immunohistochemistry was used to detect neurogenesis in the SGZ at 7, 14 and 21 d following ischemia. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) was used to detect cell apoptosis. Our results indicate that pre- and post-treatment with edaravone enhances neurogenesis and protects NSPCs from apoptosis in the hippocampus.?

keywords： neurobiology neurogenesis cerebral ischemia neural stem/progenitor cells neuroprotection edaravone

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依达拉奉对大鼠短暂性全脑缺血后海马CA1区的保护作用及对颗粒下区神经发生的影响

雷珊 ¹
LEI Shan,1986.07,female,doctoral student,the neuroprotection of cerebral ischemia
张蓬勃 ¹
ZHANG Pengbo,1968.03,male,professor,the neuroprotection and regeneration of cerebral ischemia
李卫松 ¹ 高明 ¹ 贺西京 ² 郑娟 ¹ 李旭 ¹ 王宁 ¹ 汪晓 ¹ 张军峰 ³ 祁存芳 ³ 吕海侠 ³ 陈新林 ³ 刘勇 ³

1、西安交通大学医学院第二附属医院麻醉科，西安市西五路157号，710004
2、西安交通大学医学院第二附属医院骨科，西安市西五路157号，710004
3、西安交通大学医学院神经生物研究所，西安市雁塔西路76号，710061

摘要：依达拉奉在临床上被用于急性脑梗塞的治疗，然而，其对脑缺血后海马神经发生的影响仍不清楚。本研究通过制作大鼠短暂全脑缺血模型观察缺血前后依达拉奉处理对海马颗粒下区神经干/祖细胞的影响。雄性大鼠随机分为3组：假手术组（n=15），对照组（n=15）和依达拉奉处理组（n=15）。采用5-溴脱氧尿嘧啶核苷（BrdU）标记增殖细胞。缺血后7,14,21天的神经发生采用免疫组化染色方法检测。细胞凋亡采用TUNEL染色法检测。结果显示依达拉奉能够增加海马神经发生，减少海马神经干/祖细胞凋亡。

关键词：神经生物学神经发生脑缺血神经干/祖细胞神经保护依达拉奉?????

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LEI Shan,ZHANG Pengbo,LI Weisong,et al. Pre- and post-treatment with edaravone protects CA1 hippocampus and enhances neurogenesis in the subgranular zone of dentate gyrus after transient global cerebral ischemia in rats[EB/OL]. Beijing:Sciencepaper Online[2014-01-16]. https://www.paper.edu.cn/releasepaper/content/201401-779.

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论文编号	201401-779
论文题目	依达拉奉对大鼠短暂性全脑缺血后海马CA1区的保护作用及对颗粒下区神经发生的影响
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