小分子迁移对高蛋白中间水分食品质构的影响
首发时间:2014-03-19
摘要:利用质构仪、激光共聚焦显微镜以及低场核磁共振技术,分别监测模型体系在25℃下储藏24h内的质构变化、结构变化以及弛豫时间(T2)的变化,来研究分子迁移对高蛋白中间水分食品质构的影响。实验结果表明:1、新鲜的酪蛋白酸钠(NaCN)模型体系是一个非热力学稳定体系,在储藏过程中,水和其他小分子物质(甘油、山梨醇)从体相中向未溶解的蛋白颗粒表面迁移,使体系的结构发生改变,形成交联网状结构,致使质构不断变硬;2、乳清蛋白(WPI)模型体系则均一、稳定,储藏过程中没有发生明显的分子迁移和结构变化,故保持柔软的质地;3、NaCN重水体系的NMR实验结果显示,甘油、山梨醇的迁移比水慢,一定程度上延缓了小分子物质的迁移,起到了保湿作用。由此可见,由分子迁移引起的体系结构的改变及分子流动性的减小,是高蛋白中间水分食品在储藏初期硬化的重要因素。
关键词: 高蛋白中间水分食品 硬化 分子迁移 激光共聚焦显微镜 低场核磁共振
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Effect of molecular migration on texture of high-protein intermediate-moisture foods
Abstract:The objective of this study was to evaluate the influence of molecular migration on the texture of high-protein intermediate-moisture foods. Texture analyzer, confocal laser scanning microscopy (CLSM) and low-field nuclear magnetic resonance (LF-NMR) were used to monitor the changes in texture, microstructure and T2 relaxation time of model systems during storage for 24 hours at 25℃. Sodium caseinate (NaCN) model system was thermodynamically unstable and showed significant increase in hardness during storage. Formation of cross-network and migration of low molecular weight compounds from lipid phase to insoluble protein powders were found, which may contribute to the firm texture of NaCN system. In contrast, Whey protein Isolate (WPI) model system showed little molecular migration or microstructural changes and kept soft texture all the time. NMR experiment of NaCN heavy water system showed that glycerol and sorbitol had lower mobility than water and their existence could delay moisture migration to keep the system softer. These results confirmed that molecule-migration-induced changes in microstructure and molecular mobility were the key factors of the hardening of high-protein intermediate-moisture foods.
Keywords: high-protein intermediate-moisture foods hardening molecular migration CLSM LF-NMR
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