Enhance eicosapentaenoic acid production in oleaginous fungus Mortierella alpina by overexpressing ω3 fatty acid desaturase
首发时间:2014-03-25
Abstract:ω3 fatty acid desaturase is the key enzyme responsible for ω-3 polyunsaturated fatty acids (PUFAs) formation. In oleaginous fungus Mortierella alpina, about 50% of total fatty acids are arachidonic acid (AA), whereas ω-3 PUFAs level is very low. In order to enhance the eicosapentaenoic acid (EPA) production, ω3 fatty acid desaturase gene (FADS15) from M. alpina ATCC32222 was cloned and homologously overexpressed, using the Agrobacterium tumefaciens-mediated transformation. Compared to the wild-type M. alpina, the FADS15 overexpressing strains MA-ω3Des-1, MA-ω3Des-2, MA-ω3Des-3 had 6.35-, 5.33- and 6.27-fold increase in EPA yield, respectively. These FADS15 overexpressing clones may be useful to future industrial production of EPA.
keywords: Mortierella alpina ω3 fatty acid desaturase overexpression EPA production
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高山被孢霉中过表达ω3脂肪酸脱饱和酶提高EPA产量的研究
摘要:ω3脂肪酸脱饱和酶是ω-3多不饱和脂肪酸形成过程中的关键酶。在产油微生物高山被孢霉(Mortierella alpina,M. alpina)中,花生四烯酸(AA)占菌体总脂肪酸含量的50%,而其中的ω-3多不饱和脂肪酸(如二十碳五烯酸,EPA)含量很低。为提高高山被孢霉中EPA的产量,本研究成功构建了含有ω3脂肪酸脱饱和酶基因(FADS15)的重组二元表达载体,通过根癌农杆菌介导的方法将FADS15整合至高山被孢霉的染色体,实现了ω3脂肪酸脱饱和酶的同源过表达。所获重组菌的脂肪酸含量分析结果表明,高山被孢霉重组转化子MA-ω3Des-1、MA-ω3Des-2、MA-ω3Des-3的EPA产量分别提高到了野生型对照菌株的6.35倍、5.33倍和6.27倍。此结果为将来通过基因工程的方法构建EPA高产菌提供了借鉴。
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