Chemical Composition and Enzymatic Saccharification of Poplar
首发时间:2014-04-18
Abstract:Green liquor (Na2S + Na2CO3, GL) pretreatment is an effective pathway for improving the enzymatic digestibility of lignocellulosic biomass for the production of bioethanol. In this work, GL was employed as a pretreatment to enhance the enzymatic saccharification of poplar. During pretreatment, the increase of H-factor and TTA charge resulted in enhanced delignification and increased degradation of polysaccharides. The sugar yield of enzymatic hydrolysis increased rapidly with increasing TTA charge in GL pretreatment, while the effect of different H-factors (from 400 to 800) on sugar yield was unnoticeable. The pretreated solid recovery was 75.5% at a lignin removal rate of 29.2% under optimized conditions of total titratable alkali (TTA) charge 20%, sulfidity 25%, and H-factor 400. The sugar yield of glucan, xylan, and total sugar of GL-pretreated poplar in enzymatic hydrolysis reached up to 89.9%, 65.5%, and 82.8%, respectively, at a cellulase loading of 40 FPU/g-cellulose.
keywords: Poplar Green liquor pretreatment H-factor Enzymatic hydrolysis Sugar
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绿液预处理对杨木化学成分及酶水解性能的影响
摘要:绿液(Na2S + Na2CO3, GL)预处理是一种能够有效提高部分木质纤维原料酶水解效率的预处理方法。本研究主要探讨了在不同总用碱量和H-因子的条件下绿液预处理对杨木化学成分及酶水解性能的影响。在绿液预处理过程中,随着预处理用碱量增加和H-因子上10 升,木质素的脱除率上升,但同时也加剧了碳水化合物的降解。预处理后的浆料用Cellic CTec2 复合酶进行酶水解,随着预处理用碱量和酶用量增加,各聚糖转化率上升,但H-因子(400-800)对各聚糖转化率的影响并不显著。在本试验最优条件(温度160℃,用碱量20%,硫化度25%,H 因子400)下,浆料的的得率为75.5%,木质素脱除率29.2%,当酶用量为40 FPU/g-纤维素时,葡聚糖、木聚糖和总糖的转化率分别为89.9%、65.5%和82.8%。
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