健脾除痰法治疗肺癌对顺铂增效减毒作用的研究
首发时间:2014-05-29
摘要:目的:明确中药"健脾除痰方"的抗癌疗效及与顺铂(Cisplatin) 联合使用时之增效减毒作用,并初步探讨其作用机制。方法:首先做顺铂(Cisplatin)剂量的确定实验,确定最佳的顺铂使用剂量后,再做中药健脾除痰方和顺铂的综合实验。前者以12只Lewis荷瘤C57BL/6J小鼠为对象,分为对照组, 5mg, 6mg, 7mg 每组3只,用药后每3-4天测定小鼠肿瘤体积第15天处死。后者以40只lewis荷瘤C57BL/6J小鼠为对象,分为对照组、TCM po组、Cisplatin ip组、TCM po+Cisplatin ip组、每组10只,用药后每一天测定小鼠的体重及肿瘤体积,21天后处死,取出心、肝、脾、肺、肾及肿瘤组织,称取瘤重,对各组织做成切片后行HE染色观察变化。结果:顺铂的最佳使用剂量为5mg/kg。对照组肿瘤呈指数生长趋势,其余三组较对照组均显示出抑制肿瘤生长的趋势,且具有统计学差异。在抑瘤作用方面,TCM po+ Cisplatin ip组疗效优于TCM po组及Cisplatin ip单药组(P<0.05)。在生存率方面,TCM po+ Cisplatin ip组(80.00%)均优于TCM po组(60.00%)、Cisplatin ip单药组(50.00%)、Control组(50.00%)。病理切片观察只发现脾脏变化明显. 心、肝、肺、肾等其他器官变化不明显。结论:健脾除痰方对lewis肺癌具有抑制作用,其机制可能与促进T细胞增长,提高机体免疫力相关。健脾除痰方与顺铂同时使用可提高顺铂抑瘤疗效的作用,且二者联合应用比两药单用能明显提高存活率,其机制可能与中药健脾除痰方可减轻顺铂抑制免疫功能的副作用有关。
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The Prospective Study and Prognosis Analysis of NSCLC Treated by
Abstract: Objective: To disclose the anti-cancer effects of TCM "Invigorating Spleen & Phlegm Elimination" and its effectiveness to reduce the cisplatin toxicity and increase survival rate of the cisplatin's treatment. Method:40 mice C57BL/6J implanted with Lewis tumor cell divided into the Control group, TCM group, cisplatin group, TCM + cisplatin group, with 10 members each. After drug treatment the mice are examined and measured for tumor size and body weight every day and terminated on the 21st day. The hearts, livers, lungs, spleens, kidneys, and cancerous tissues are removed, weighed, sectioned and dyed with HE in order to observe changes. Results: The tumors in the control group show an exponential growth trend,and the remaining three groups compared with the control group showed a trend to inhibit tumor growth, and has a statistically significant difference. The antitumor effect of TCM po+ Cisplatin ip group is superior to TCM po group, and Cisplatin ip group. The changes in spleen are readily apparent, while in other organs not apparent under the microscope.Conclusion:TCM "Invigorating Spleen & Phlegm Elimination" has suppressive effects on Lewis lung cancer, and can increase survival rate. The mechanism may involve promoting T-cell regeneration, and increasing the body's immune capabilities. The combination treatment with TCM "Invigorating Spleen & Phlegm Elimination" and Cisplatin improve Cisplatin's tumor-suppression effects, and the combination treatment clearly increased survival rate beyond that of using each drug alone. TCM alleviating Cisplatin's immuno-suppressive effects is a possible mechanism.
Keywords: Non-Small-Cell Lung Cancer (NSCLC) Cisplatin TCM (Traditional Chinese Medicine) Invigorating Spleen, Vital Engergy & Phlegm Elimination
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