Isolation and Sequence Analysis of bHLH Transcription Factor Genes in Tree Peony

• 1、College of Landscape Architecture, Beijing Forestry University, National Flower Engineering Technology Research Center, Beijing 100083

Abstract：In this study, two Unigene sequences that share high homology with WD40 protein involved in plant anthocyanin biosynthesis were obtained from previous-constructed tree peony (Paeonia suffruticosa 'Luoyang Hong') petal transcriptome database and named as PsWD40-1 and PsWD40-2. Sequences of Open Reading Frame (ORF) in PsWD40-1 and PsWD40-2 were amplified with designed specific primers using RT-PCR technology and sequenced. Results showed that PsWD40-1 contains a 1035 bp ORF encoding 344 amino acid residues, and PsWD40-2 contains a 1032 bp ORF encoding 343 amino acid residues. The predicted protein sequences of PsWD40-1 and PsWD40-2 genes both contain the typical WD40 structural domain. PsWD40-1 shares high similarity (96%) with VvWDR2 of Vitis vinifera, and PsWD40-2 shares 87% similarity with VvWDR1 of Vitis vinifera. Phylogenetic tree analysis showed that PsWD40-1 is grouped into the MP1 clade with VvWDR2 of Vitis vinifera, AtAN11 of Arabidopsis thaliana, and GhTTG2 of Gossypium hirsutum, while PsWD40-2 is grouped into the other clade, TTG1/PAC1 clade, with VvWDR1 of Vitis vinifera, PhAN11 of Petunia hybrida, and PfWD40 of Perilla frutescens.

Keywords： Landscape plant and ornamental horticulture anthocyanin biosynthesis regulatory gene Paeonia suffruticosa