UV-B辐射下不同硅吸收水稻的PAL酶活及其基因家族表达变化研究
首发时间:2014-06-11
摘要:以Lsi1基因分别被过量表达和RNA干扰的转基因Lemont水稻、Lsi1过量表达的转基因Dular水稻,及其相应的野生型植株为材料,通过分析比较不同Lsi1表达丰度的转基因水稻及其野生型植株的苯丙氨酸解氨酶(PAL)的活性及总酚、类黄酮的含量,结果显示,过量表达水稻的Lsi1后,其叶片的PAL酶活及总酚、类黄酮含量显著增加,有利于提高水稻的抗逆能力;抑制水稻Lsi1表达,则水稻上述酶活性显著降低,总酚、类黄酮含量显著下降,水稻防御能力下降。进一步对两种转基因水稻及其野生型植株进行UV-B辐射处理,并比较上述指标在水稻中的变化,结果显示,UV-B辐射下,Lsi1过量表达水稻的酶活性仍显著高于野生型植株,总酚、类黄酮含量也显著高于野生型植株;但Lsi1被抑制的水稻中的上述指标均与Lsi1过量表达水稻中的相应指标相反,研究结果暗示Lsi1能够通过调控水稻的生理代谢来提高水稻的耐UV-B辐射。PAL是一个重要的防御调控相关的生理酶,其能够调控总酚、类黄酮等提高植物防御UV-B的次生物质,编码该酶的PAL基因是一个含有11个基因成员的基因家族,利用定量PCR(qPCR)比较上述不同水稻在UV-B辐射下,这11个基因成员的表达变化,结果显示UV-B辐射下,Lsi1能够通过正调控PAL8来调控水稻的总酚、类黄酮等物质的合成。
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Study on the change PAL activity and its multigene expression from different silicon absorbing rice under UV-B radiation
Abstract:In this paper, Lsi1 overexpression transgenic Lemont, Lsi1 RNA interference transgenic Lemont, Lsi1 overexpression transgenic Dular and their wild types(WT) were used as donor plants. Change of the activity of phenylalnine ammonialyase(PAL) and the content of total polyphenols, flavonoids in different transgenic rice plants and their wild type plants were studied, the results showed that the enzyme activity of PAL was significantly improved in the Lsi1-overexpression rice leaf, and the contents of total phenol and flavonoids were significantly increased, of which is contribute to enhance rice resistance; however, the reverse was true in that of Lsi1-RNAi transgenic rice.Supplement of UV-B radiation resulted in increased enzyme activity, total phenol and flavonoids content in each rice, but the Lsi1-overexpression transgenic rice still presented highest enzyme activity, total phenol and flavonoids content, all of which contribute to enhance rice UV-B resistance, but in contrast to this, Lsi1-RNAi transgenic rice was shown weakest in UV-B resistance. PAL is an important enzyme which can control the synthesis of secondary metabolites such as the total phenol and flavonoids. The gene encoding PAL is a multigene family which contains 11 gene members. Quantitative PCR(qPCR) was then used to detect the different expression level of the 11 gene members, it was found that PAL8 could be positively regulated by Lsi1, according to compare the changes gene expression levels of the 11 members, the results suggested that Lsi1 regulates the synthesis of the substances such as total phenol and flavonoids by up-regulating the gene expression level of PAL8.
Keywords: Rice UV-B stress Low silicon gene 1 (Lsi1) silicon
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UV-B辐射下不同硅吸收水稻的PAL酶活及其基因家族表达变化研究
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