穿心莲内生真菌的分离鉴定及菌库的建立
首发时间:2014-06-11
摘要:本研究利用组织分离法从传统中药穿心莲的一千余个组织块中分离获得了49株内生真菌,并将其保存在-80℃冰箱中。进一步通过ITS序列比对和菌落形态、显微观察确定内生真菌有米曲霉、阿氏丝孢酵母、产黄青霉菌、黄曲霉、木贼镰孢菌等种类。本研究证明了穿心莲内生菌种类的多样性和丰富性,为内生菌资源的研究和进一步利用打下了基础。
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The foundation of endophytic fungi from andrographis paniculata
Abstract:In this paper, 49 endophytic fungi were isolated from more than a thousand tissue blocks of traditional Chinese medicine andrographis paniculata with the tissue separation method and these fungi were stored at -80℃. According to the resulats of ITS sequence alignment, microscopic and morphology observation, these endophytic fungi include many known strains, such as Aspergillus oryzae,Trichosporon asahii, Penicillium chrysogenum,Aspergillus flavus , and Fusarium equiseti. This study not only demonstrates the diversity and abundance of the endophytes species of andrographis paniculata, but also lays the foundation for the further research and utilization of endophytes resources.
Keywords: Endophytic Fungi Andrographis Paniculata Screening and Identification
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No.4599499873204140****
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