毛细管电泳法鉴别天麻品种
首发时间:2014-06-12
摘要:发现一种简单、明确的方法,可用于鉴别目前常见的五种天麻。采用超声波水提得待测液;在230nm检测波长,磷酸盐缓冲液稀释5倍后使用,20 KV电压等条件下进行毛细管电泳;所得图谱可见4个较大峰,其中各个品种的1、2、3号峰目测一致,4号峰具有明显的品种特异性,目测即可明显鉴别。
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Identification of Gastrodia elata Bl. by Capillary electrophoresis
Abstract:In this paper, a simple, clear method was found. It may be used to identify the five kinds of Gastrodia elata Bl. Menthods: Ultrasonic-assisted extraction of water-solvlable from Gastrodia elata Blume, detection wavelength of 230nm, 5 times diluted phosphate buffer, and 20 KV voltage. Maps in capillary zone electrophoresis are typically obtained in 4 large peaks, various of 1, 2, 3, 4th, and the 4th peak has a significant species specificity.
Keywords: Chinese Materia Medica Gastrodia elata Bl. Breed discrimination
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No.4599817979456140****
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