巨菌草诱导培养基的筛选及优化
首发时间:2014-10-24
摘要:巨菌草(Pennisetum sp.)隶属单子叶禾本科狼尾草属植物,可作为动物饲料和多种食用菌的培养料,也是一种生物能源,在中国及非洲广泛种植,然而其不耐寒的特性限制了其推广,传统育种方法无法解决此难题。为改良这个特性,必须借助转基因或体细胞杂交等技术,因此必须建立巨菌草的组织培养体系。本文以巨菌草幼茎为材料,研究了巨菌草外植体的消毒方式和2,4-D、IAA与NAA,细胞分裂素(6-BA和KT)的不同浓度及组合对愈伤组织诱导的影响。结果表明,用75%酒精层层擦拭剥离后的叶鞘,再用2%的次氯酸钠溶液消毒10min就能达到理想的消毒目的。1.0mg/L~4.0mg/L的2,4-D都可以诱导出愈伤组织,且没有显著差异(P<0.05);0.2mg/L IAA对愈伤组织诱导有较好的促进作用;不同细胞分裂素对愈伤组织诱导没有明显促进作用。巨菌草愈伤组织诱导的最佳培养基配方是MS+2,4-D(3.0mg/L)+IAA(0.2mg/L)。
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Screening and optimization of Pennisetum sp. induction medium
Abstract:Pennisetum sp. is a monocotyledon gramineae penniseetum plant, which can be used as animal feed and a substrates for a variety of edible fungi. It has been widely planted in China and Africa. However it is not tolerant to cold, which limits its popularization. The traditional breeding couldn't solve this problem. In order to improve the cold tolerance or quality of Pennisetum sp., transgenic technology or somatic hybridization can be used. Therefore it is urgent to establish the tissue culture system for Pennisetum sp. In this article, we use the caulicle of Pennisetum sp. as material to research the disinfection way of explants and proper concertration of different growth hormone such as 2,4-D, IAA, NAA and cytokinins(6-BA and KT) used in induction medium. The result shows that the ideal disinfection method is to use 75% alcohol to wipe the leaf sheath after stripping layer upon layer, and then soak the leaf sheath into 2% sodium hypochlorite solution for 10 min. The concentration of 2,4-D from 1.0mg/L to 4.0mg/L all can induce callus,and there is no significant difference;0.2mg/L IAA can promot the development of callus induction;different cytokinins can not improve the callus obviously.The optimal callus induction medium of Pennisetum sp. is MS + 2,4-D (3.0mg/L) + IAA(0.2mg/L) .
Keywords: Pennisetum sp. Caulicle Callus Induction Medium
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