普通小麦Amy1基因克隆与序列分析
首发时间:2014-10-09
摘要:α-淀粉酶对小麦穗发芽的影响较大,较高的α-淀粉酶活性能促使小麦穗发芽的发生,对α-淀粉酶合成基因的等位变异分析有利于深入研究α-淀粉酶对小麦穗发芽的影响。本研究通过对不同地域来源的15份小麦材料进行穗发芽抗性分析和Amy1基因进行克隆测序。结果获得1份抗性种质资源,2份较抗穗发芽材料,同时也分析了Amy1基因在这些地理差异较大的小麦中的等位变异位点,在编码区获得50个稳定的SNP变异位点,获得两种单倍型,另外在编码区还存在较低频率的变异位点,这些位点可以作为潜在的SNP位点,对以后进一步研究Amy1基因做铺垫。结合穗发芽抗性鉴定结果和Amy1基因的SNP位点分析发现,在抗性材料中,Amy1基因第2个内含子中存在4bp的特异片段缺失(TCAT),推测该片段为抗性相关位点。可用于开发特异标记,并用于穗发芽抗性分子育种。
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Cloning and Sequence Analysis of Amy1 gene in Common Wheat, Triticum aestivum
Abstract:Alpha-amylase is closely associated with pre-harvest sprouting in wheat. High alpha-amylase activity would result in serious damage of pre-harvest sprouting. Analysis of allelic variation of Amy1 gene would be helpful to further study the influence of alpha-amylase to pre-harvest sprouting. This study identified GI value of 15 wheat accessions and found the 50 stable variation sites of Amy1 genes coding domains by cloning and sequencing Amy1 genes from 15 wheat accessions collected from different regions in the world. Furthermore, two haplotypes was also found, and a few variation sites with low frequency were found, which was regarded as the potential SNPs to contribute to the further study of Amy1 gene in wheat. We also found a special deletion element in Intron II among two resist wheat accessions, which was TCAT. This allelic variation would be used to molecular breeding of resistance for pre-harvest sprouting in wheat.
Keywords: wheat alpha-amylase Amy1 gene allelic variation
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