Preparation and characterization of mPEG-b-PLGA-b-PEI nano micelles with pEGFP-ZNF580

• 1、Collaborative Innovation Center of Chemical Science and Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

Abstract：With the rapid development of gene therapy, cationic polymers as gene transfer vectors have caused much attention and a large amount of researches have focused on this field. Polyethylene imine (PEI), as a kind of off-the-shelf material, has become one of the most promising gene vectors. pEGFP-ZNF580 plasmid can promote the growth of endothelial cells and is conducive to the therapy of vascular diseases. Herein, we prepared a kind of triblock copolymer mPEG-b-PLGA-b-PEI, which can self-assemble into micelles as transfer vector for pEGFP-ZNF580 plasmid. PLGA, as a kind of medical biodegradable material, can control plasmid release rate. The results of size and potential showed that before loading plasmid, the average diameter of the particles was about 89 nm and the potential was 13.27 mV; after loading plasmid, the particle size was reduced to about 60 nm, potential became negative. These nanosized particles are stable, and have a certain potential as a gene vector and for the therapy of vascular diseases.

Keywords： PLGA pEGFP-ZNF580 plasmid gene delivery vector polyethylene imine