Preparation of chloramphenical molecularly imprinted polymers and SERS detection

• 1、School of Food Science and Technology,Jiangnan University,wuxi 214122

Abstract：This study combined molecular imprinting technology (MIP) and surface-enhanced Raman spectroscopy (SRES) detection technology, integrated both the advantages of high sensitivity and specificity ,intends to establish a new method for fast trace detection of chloramphenicol .We will prepare the chloramphenicol molecularly imprinted polymers and synthetic chloramphenicol MIP/Au substrateand,and apply it in SERS detection of chloramphenicol. The chloramphenicol molecularly imprinted polymers was prepared by precipitation polymerization,and use the ultrasound-assisted extraction technology to elute template molecule, and get the chloramphenicol molecularly imprinted polymers. The polymer was placed in sodium citrate reduction reaction to prepare the chloramphenicol MIP / Au substrate. The substrate fully enriched 6h in methanol: water(1:9) chloramphenicol liquor , with good detection results can be applied to the detection of chloramphenicol in milk samples, the detection limit is 0.1ppm. This study provides a new method for trace amounts of chloramphenicol fast detecting, it can be directly used for chloramphenicol enrichment and detection in food samples, simplifying the detection step, to improve the detection efficiency, has broad application prospects.

Keywords： Chloramphenicol molecular imprinting SERS rapid detection