新型黑曲霉固定化细胞的制备及其对黄曲霉毒素B1的降解
首发时间:2014-12-17
摘要:采用紫外线照射诱变与酪蛋白培养基对黑曲霉进复合选育,最终获得对黄曲霉毒素B1降解率较高的菌株,CCTCC对该菌株进行18S rDNA测序并命名为FS-UV1。利用FS-UV1与苏州当地生产的蒙脱石制备固定化细胞,研究了最佳制备条件及对发酵液中黄曲霉毒素B1的吸附降解效果。结果表明:紫外照射17 min 时,产生的突变率最高,经复筛菌株对黄曲霉毒素B1的降解率最高可达65.98 %。固定化细胞最佳制备条件为:pH=10,40 ℃,蒙脱石含量0.1%(w:v)磁力搅拌30min。在此条件下制备的固定化细胞对发酵液中黄曲霉毒素B1吸附降解率最高可达到94.49%。?????
关键词: 黑曲霉 诱变 固定化细胞 黄曲霉毒素B1 生物降解
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Aflatoxin B1 Decontamination by UV-Mutated Immobilized
Abstract:In this paper the decontamination of Aflatoxin B1 (AFB1) by immobilized cells of a new mutant strain was studied, which were prepared on a base of montmorillonite in Suzhou. The mutant strains were obtained by UV irradiation and Casei medium. The Strain of FS-UV1 showed highest degradation effect, which was confirmed to be Aspergillus niger by 18SrDNA. Our results indicate immobilized cells and this mutant strain irradiated for 17 min by UV and incubated for 48 h at 30℃ considerably remediate AFB1 in nutrient broth culture, by 94.49 % and 65.98 % respectively. The results showed that the ideal parameters are: pH 4 at 40 ℃, reacting for 30min, with 0.1 % clay concentration.
Keywords: Aspergillus niger Mutant strain Immobilized cells AFB1 Biodegradation
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No.4623009102421914****
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