Accumulation and in situ detection of 1-hyroxypyrene by differential pulse adsorptive cathodic stripping voltammetry

• 1、Food Science And Technology School, Jiangnan University, WuXi 214122
• 2、Food Science and Technology School,Jiangnan University,Wuxi 214122

Abstract：In this paper, a sensitive and fast electrochemical approach was developed for the accumulation and in situ detection of 1-hyroxypyrene (1-OHP). Electro-reduced grapheme oxide (ErGO) was directly deposited on glass carbon electrode (GCE) by eletrochemical reduction of GO to obtain an ERGO-modified electrode. Scanning electron microscope was used to characterize the modified electrode. The electrochemical behaviors of 1-hyroxypyrene (l-OHP) on the modified electrode were investigated by cyclic voltammetry. Based on this, a novel method of adsorptive cathodic stripping voltammetry (DPCSV) for 1-hyroxypyrene accumulation and in situ detection was fabricated. Under the optimal conditions, linear responses were obtained between the reduction current of 1-OHP and the concentration of 1-OHP ranged from 5 nM to 300 nM (R2=0.997), with a limit of detection of 0.84 nM (S/N=3) and a sensitivity of 0.1684 μAoμM-1. The relative standard deviation is 4.6% for determination of 300nM 1-OHP for 5 times. Finally, it was successfully applied to the determination of 1-OHP in real human urine samples with recoveries of 97.3 ~ 101.1%.

Keywords： 1-hyroxypyrene polycyclic aromatic hydrocarbons accumulation and electrochemical detection in situ electro-reduced grapheme oxide