QCM-D在纤维素酶水解研究中的应用
首发时间:2015-01-08
摘要:耗散型石英晶体微天平(QCM-D)是基于石英的压电特性制备的一种表面敏感型分析技术,能感应到纳克级的质量变化。酶水解是纤维素生物转化的重要途径,准确表征在酶水解过程中酶的吸附、解吸及其与纤维素的降解动力学的关系,对制订合理的技术途径、提高纤维素的酶水解效率、降低转化成本具有重要的指导意义。传统化学方法不能实时反映酶水解的动态过程及其动力学特征。通过QCM-D在线测定酶水解过程中频率和能量耗散变化,可原位、实时响应水解底物表面的质量变化,直观地反映纤维素酶在底物上的吸附、解吸,以及纤维素酶水解的动态过程。概述了QCM-D的原理和其生物传感器的制备,以及近几年QCM-D技术应用于纤维素酶水解研究的进展。
关键词: 耗散型石英晶体微天平(QCM-D) 纤维素 生物传感器 吸附与解吸 酶水解动力学
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The application of QCM-D in the research on enzymatic hydrolysis of cellulose
Abstract:QCM-D (Quartz crystal microbalance with dissipation) is a surface-sensitive analytical technique based on the piezoelectric properties of quartz crystal and it senses the mass change in nanoscale. Enzymatic hydrolysis is a vital approach for the bioconversion of cellulosic materials, and accurate characterization of the adsorption, desorption of enzymes on the substrates as well as the relationship between them and the kinetics of cellulosic digestion is full of guiding significance. These accurate characterizations help draw up appropriate technological approaches, improve the efficiency of enzymatic hydrolysis of cellulose and reduce the cost of biotransformation. Traditionally, chemical analysis methods are used mainly through quantitative analysis of sugar yields or the changes of enzyme concentration in hydrolysate so as to indirectly evaluate the effect of adsorption, desorption and enzyme digestion on the enzymatic hydrolysis, which fails to reflect the whole dynamic process and the features of kinetics of enzymatic hydrolysis online. QCM-D gives the information in situ and response to the surface quality changes on the substrates on top of quartz crystal in real-time through the variations of frequency and dissipation, so that the adsorption and desorption of enzymes on the substrate, as well as the dynamic enzymatic hydrolysis process can be visually analyzed. In this article, the operating principle of QCM-D and the preparation methods of the biosensor, as well as the latest developments of the application of QCM-D on the research of enzymatic hydrolysis of cellulosic materials were introduced.
Keywords: QCM-D cellulose biosensor adsorption and desorption kinetics of enzymatic hydrolysis
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