差分脉冲伏安法原位富集传感1-羟基芘
首发时间:2015-01-15
摘要:本文建立一种快速灵敏的原位富集电化学传感1-羟基芘(1-hyroxypyrene, l-OHP)方法。采用电化学还原法制备还原氧化石墨烯修饰电极(electro-reduced grapheme oxide modified electrode, E-rGO/GCE)。利用扫描电镜对该修饰电极进行形貌表征。采用循环伏安法研究1-OHP在修饰电极上的电化学行为,并在此基础上建立一种通过差分脉冲伏安法原位富集传感1-OHP的新方法。1-OHP在5 ~ 300 nM浓度范围内与其还原峰电流呈良好的关系,相关系数为0.997,检出限为0.84 nM (S/N=3),灵敏度为0.1684 μA﹒μM-1。对300 nM 1-OHP平行测定5次,其相对标准偏差为4.6%。将该方法用于人体尿液中1-OHP含量的测定,回收率为97.3 ~ 101.1%,结果令人满意。
关键词: 1-羟基芘 多环芳烃 原位富集电化学传感 电还原氧化石墨烯
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Accumulation and detection of 1-hyroxypyrene in situ by differential pulse voltammetry
Abstract:In this paper, a sensitive and fast electrochemical approach was developed for the accumulation and in situ detection of 1-hyroxypyrene (1-OHP). Electro-reduced grapheme oxide (E-rGO) was directly deposited on glass carbon electrode (GCE) by eletrochemical reduction of GO to obtain an E-rGO modified electrode. Scanning electron microscope was used to characterize the modified electrode. The electrochemical behaviors of 1-hyroxypyrene (l-OHP) on the modified electrode were investigated by cyclic voltammetry (CV). Based on this, a novel method of differential pulse voltammetry (DPV) for 1-hyroxypyrene accumulation and in situ detection was fabricated. Under the optimal conditions, linear responses were obtained between the reduction current of 1-OHP and the concentration of 1-OHP ranged from 5 nM to 300 nM (R2=0.997), with a limit of detection of 0.84 nM (S/N=3) and a sensitivity of 0.1684 μA﹒μM-1. The relative standard deviation is 4.6% for determination of 300nM 1-OHP for 5 times. Finally, it was successfully applied to the determination of 1-OHP in real human urine samples with recoveries of 97.3 ~ 101.1%.
Keywords: 1-hyroxypyrene polycyclic aromatic hydrocarbons accumulation and electrochemical detection in situ electro-reduced grapheme oxide
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